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Using high throughput tandem mass tag (TMT) based tagging technique, we identified 4172 proteins in three developmental stages: early, mid, and late seed filling. We mapped the identified proteins to metabolic pathways associated with seed filling. The elevated abundance of several kinases was observed from the early to mid‐stages of seed filling, indicating that protein phosphorylation was a significant...
To understand the effect of fatty acid desaturase gene (GmFAD3) silencing on perturbation of fatty acid (FA) metabolic pathways, the changes are compared in protein profiling in control and low linolenic acid transgenic soybeans using tandem mass tag based mass spectrometry. Protein profiling of the transgenic line unveiled changes in several key enzymes of FA metabolism. This includes enzymes of...
DOI: 10.1002/pmic.201800379
In article number 1800379, Nazrul Islam et al. compare protein profile changes in normal and low linolenic acid transgenic soybeans using high‐throughput tandem mass tag (TMT) based mass spectrometry. Proteomic analysis of the transgenic line unveils changes in several key enzymes involved in fatty acid metabolism. These results will be important for the understanding how...
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