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Total proteins from Apios americana Medikus tubers were resolved by SDS-PAGE into several abundant polypeptides of approximate molecular masses of 55, 42, 29, 22 and 14ku (kDa). These proteins accumulated in a coordinate fashion during tuber development. We purified the 29 ku protein by ammonium sulfate precipitation, extraction with 70 % ethanol, and affinity chromatography on Con-A Sepharose. The...
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