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d-Galactose was produced from lactose (200gl −1 ) in the batch mode of a simultaneous saccharification and fermentation process (SSF). β-Galactosidases (from Kluyveromyces lactis and Aspergillus oryzae) and yeasts were immobilized in poly(vinylalcohol) hydrogel lens-shaped capsules – LentiKats ® . After 20 repeated batch runs with entrapped K. lactis, β-galactosidase and free Saccharomyces...
Invertase was immobilised into polyvinyl alcohol capsules (LentiKats ® ). The enzyme retained 86% of its original activity after the immobilisation process. Kinetic constants, pH and temperature profiles, and stability were determined, for both free and immobilised enzyme. Entrapped invertase was used for 45 repeated batch hydrolyses and no decrease in enzymatic activity was observed. The...
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