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Ultrasound biomicroscopy (UBM)-Doppler allows study of cardiovascular physiology in the in utero mouse embryo from embryonic day (E)8.25 onward. We determined the accuracy of localization of embryos by transabdominal, noninvasive 40-MHz UBM-Doppler imaging. Nuclear factor of activated T cells c1−/− mice lack semilunar valves, exhibit outflow tract regurgitation, and die in utero. In timed pregnant...
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