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Measurement of free estradiol offers a better representation of the bioactive fraction of the hormone. We describe a direct equilibrium dialysis–liquid chromatography–tandem mass spectrometry (ED–LC–MS/MS) method for serum free estradiol.Two hundred fifty microliter aliquots of serum were dialyzed for 22h followed by liquid–liquid extraction and derivatization with dansyl chloride. Free estradiol...
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