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This study described a sensitive direct competitive enzyme-linked immunosorbent assay (ELISA) for naked-eye detection of aflatoxin B1 (AFB1) by using glucose oxidase (GOx)-regulated bromocresol purple (BCP) color change. GOx was used as an alternative to horseradish peroxidase (HRP) for oxidization of glucose into hydrogen peroxide and gluconic acid. BCP, whose color is significantly sensitive to...
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