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This study is through the Recombinant PCR reaction and restriction enzyme digestion connection to construct ginseng CS RNA interference plant expression vector, was transformed by heat into the Agro bacterium rhizogenes A4 to get CS RNA interference plant expression vector engineered bacteria, and lay the foundation for further induced transformation of ginseng explants.
The gene encoding 34kDa protein gene from Mycobacterium paratuberculosis C-2, chromosomal DNA was amplified by using polymerase chain reaction (PCR), the PCR product was approximately 897bp DNA segment. The PCR product was cloned into pGEM-T vector and the cloning plasmid pGEM-T-34 was constructed successfully. The purified 34kDa protein gene was subcloned into the expression vector pET28a(+), and...
The gene encoding ESAT-6 from Mycobacteriumbovis Vallee111 chromosomal DNA was amplified by using polymerase chain reaction (PCR), the PCR product was approximately 288bp DNA segment. The PCR product was cloned into pGEM-T vector and the cloning plasmid pGEM-T-ESAT-6 was constructed successfully. The purified ESAT-6 gene was subcloned into the expression vector pGEX-4T-3, and the prokaryotic expression...
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