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A 1.020-bp esterase gene, estQ, encoding for a protein of 339 amino acids, was cloned from Aspergillus fumigatus and expressed in E. coli. EstQ exhibited the optimal activity around 40°C and pH 9.0. In order to obtain more thermostable esterases, three mutants (A134T, V160T, A134T–V160T) were constructed by site-directed mutagenesis and also characterized for further research. Compared to A134T and...
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