Search results for: Dudley H. Williams

Items from 1 to 20 out of 35 results

article

Enzyme catalysis from improved packing in their transition-state structures

Dudley H Williams

Current Opinion in Chemical Biology > 2010 > 14 > 5 > 666-670

The binding of ligands to proteins can be enhanced through improved packing within the proteins that may, or may not, occur with conformational change. Enzymes can similarly improve their catalytic magic through better packing in the transition state (TS) for reaction. In principle, the improved packing demands no more than the minute shortening of non-covalent interactions throughout much of the...

article

Ligand Binding Energy and Enzyme Efficiency from Reductions in Protein Dynamics

Dudley H. Williams, Min Zhou, Elaine Stephens

Journal of Molecular Biology > 2006 > 355 > 4 > 760-767

Tetrameric rabbit muscle glyceraldehyde 3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) binds successively four molecules of its cofactor (NAD ⁺ ) with affinities of ca 10 ¹¹ M ⁻¹ , 10 ⁹ M ⁻¹ , 10 ⁷ M ⁻¹ , and 10 ⁵ M ⁻¹ . The reduction in the dynamics of the protein is greatest upon binding the first NAD ⁺ molecule. Smaller...

article

Importance of Structural Tightening, as Opposed to Partially Bound States, in the Determination of Chemical Shift Changes at Noncovalently Bonded Interfaces

Dudley H. Williams, Nichola L. Davies, Jari J. Koivisto

Journal of the American Chemical Society > 2004 > 126 > 43 > 14267 - 14272

article

Order Changes within Receptor Systems upon Ligand Binding: Receptor Tightening/Oligomerisation and the Interpretation of Binding Parameters

Dudley H. Williams, Dominic P. O'Brien, Alan M. Sandercock, Elaine Stephens

Journal of Molecular Biology > 2004 > 340 > 2 > 373-383

Recent hydrogen-deuterium exchange experiments have highlighted tightening and loosening of protein structures upon ligand binding, with changes in bonding (ΔH) and order (ΔS) which contribute to the overall thermodynamics of ligand binding. Tightening and loosening show that ligand binding respectively stabilises or destabilises the internal structure of the protein, i.e. it shows positive or negative...

article

Fragmentation Characteristics of Neutral N-Linked Glycans Using a MALDI-TOF/TOF Tandem Mass Spectrometer

Elaine Stephens, Sarah L. Maslen, Luke G. Green, Dudley H. Williams

Analytical Chemistry > 2004 > 76 > 8 > 2343 - 2354

article

Noncovalent Interactions: Defining Cooperativity. Ligand Binding Aided by Reduced Dynamic Behavior of Receptors. Binding of Bacterial Cell Wall Analogues to Ristocetin A

Dudley H. Williams, Nichola L. Davies, Rosa Zerella, Ben Bardsley

Journal of the American Chemical Society > 2004 > 126 > 7 > 2042 - 2049

article

Ligand Binding Energy and Catalytic Efficiency from Improved Packing within Receptors and Enzymes

Dudley H. Williams, Elaine Stephens, Min Zhou

Journal of Molecular Biology > 2003 > 329 > 2 > 389-399

Some small molecules bind to their receptors, and transition states to enzymes, so strongly as to defy current understanding. We show that in the binding of biotin to streptavidin, the streptavidin structure becomes better packed. We conclude that this contraction of the streptavidin structure promotes biotin binding. The improved packing is associated with positively cooperative binding, occurring...

article

Isolation and Structure Elucidation of Chlorofusin, a Novel p53-MDM2 Antagonist from a Fusarium sp. [ J. Am. Chem. Soc. 2001 , 123 , 554−560].

Sara J. Duncan, Sabine Grüschow, Dudley H. Williams, Carole McNicholas, more

Journal of the American Chemical Society > 2002 > 124 > 48 > 14503 - 14503

article

Cooperative Binding Interactions of Glycopeptide Antibiotics

Hideyuki Shiozawa, Brian C. S. Chia, Nichola L. Davies, Rosa Zerella, more

Journal of the American Chemical Society > 2002 > 124 > 15 > 3914 - 3919

article

On the biosynthesis of an inhibitor of the p53/MDM2 interaction

Sara J. Duncan, Dudley H. Williams, Martyn Ainsworth, Steven Martin, more

Tetrahedron Letters > 2002 > 43 > 6 > 1075 - 1078

The biosynthesis of a fungal secondary metabolite, chlorofusin, which disrupts the interaction between the proteins p53 and MDM2, has been investigated; the acetogenic origin of the chromophore backbone as well as of an aminodecanoic acid residue is demonstrated.

article

An Enthalpic Component in Cooperativity: The Relationship between Enthalpy, Entropy, and Noncovalent Structure in Weak Associations

Christopher T. Calderone, Dudley H. Williams

Journal of the American Chemical Society > 2001 > 123 > 26 > 6262 - 6267

article

Enthalpy/Entropy Compensation as a Competition between Dynamics and Bonding: The Relevance to Melting of Crystals and Biological Aggregates

Dudley H. Williams, Dominic P. O'Brien, Ben Bardsley

Journal of the American Chemical Society > 2001 > 123 > 4 > 737 - 738

article

Isolation and Structure Elucidation of Chlorofusin, a Novel p53-MDM2 Antagonist from a Fusarium sp.

Sara J. Duncan, Sabine Grüschow, Dudley H. Williams, Carole McNicholas, more

Journal of the American Chemical Society > 2001 > 123 > 4 > 554 - 560

article

Binding of vancomycin group antibiotics to d-alanine and d-lactate presenting self-assembled monolayers

Matthew A. Cooper, Maria T. Fiorini, Chris Abell, Dudley H. Williams

Bioorganic & Medicinal Chemistry > 2000 > 8 > 11 > 2609-2616

Peptides terminating in -Lys-d-Ala-d-Ala, -Lys-d-Ala-l-Ala and -Lys-d-Ala-d-Lactate were covalently coupled via an N-terminal aminohexanoic acid linker to a self-assembled monolayer of HS(CH₂)₁₅CO₂H on a thin gold film. Binding of the glycopeptide antibiotics vancomycin and chloroeremomycin to these surfaces was then measured using a surface plasmon resonance biosensor. Both antibiotics bound with...

article

A Vesicle Capture Sensor Chip for Kinetic Analysis of Interactions with Membrane-Bound Receptors

Matthew A. Cooper, Anna Hansson, Stefan Löfås, Dudley H. Williams

Analytical Biochemistry > 2000 > 277 > 2 > 196-205

A novel sensor chip for use in surface plasmon resonance (SPR) biosensors has been developed to capture vesicles which may contain membrane-bound receptors. Sulforhodamine-containing vesicles were shown by fluorescence microscopy to be immobilized intact on the sensor chip. Binding of cholera toxin to captured vesicles containing ganglioside GM ₁ was demonstrated using SPR, and the derived...

article

Binding of glycopeptide antibiotics to a model of a vancomycin-resistant bacterium

Matthew A Cooper, Dudley H Williams

Chemistry & Biology > 1999 > 6 > 12 > 891-899

Background: The vancomycin group of glycopeptide antibiotics is active against a wide range of gram-positive bacteria. The increasing resistance to vancomycin is the result of a change of an amide linkage (d-Ala-d-Ala) to an ester linkage (d-Ala-d-Lactate) in the bacterial cell-wall precursors. Results: We have used a peptide terminating in the sequence -Lys-d-Ala-d-Lactate linked by its amino terminus...

article

Kinetic Analysis of Antibody-Antigen Interactions at a Supported Lipid Monolayer

Matthew A. Cooper, Dudley H. Williams

Analytical Biochemistry > 1999 > 276 > 1 > 36-47

Modified phospholipids possessing carboxyl head groups synthesized from phosphatidylethanolamine were incorporated into supported lipid monolayers on top of a thin gold film. A monoclonal antibody was chemically coupled to the modified lipids in these monolayers and the kinetics of antigen binding were determined by surface plasmon resonance. The binding could be analyzed using a conventional 1:1...

article

Estimating binding constants – The hydrophobic effect and cooperativity

Dudley H. Williams, Ben Bardsley

Perspectives in Drug Discovery and Design > 1999 > 17 > 1 > 43-59

The reliable estimation of binding constants by computational means for drugs binding to receptors represents one of the major challenges in molecular recognition. Many approaches to this problem have relied on the partitioning of binding free energy according to each of the interactions which are made or broken on binding, e.g., hydrogen bonds, salt bridges, π-π interactions, or the hydrophobic effect...

article

Surface plasmon resonance analysis at a supported lipid monolayer

Matthew A. Cooper, Andrew C. Try, Joe Carroll, David J. Ellar, more

BBA - Biomembranes > 1998 > 1373 > 1 > 101-111

Methods for the formation of supported lipid monolayers on top of a hydrophobic self assembled monolayer in a surface plasmon resonance instrument are described. Small unilamellar vesicles absorb spontaneously to the surface of the hydrophobic self-assembled monolayer to form a surface which resembles the surface of a cellular membrane. Lipophilic ligands, such as small acylated peptides or glycosylphosphatidylinositol-anchored...

article

Synthesis of Covalent Head-to-Tail Dimers of Vancomycin

Thomas Staroske, Dudley H. Williams

Tetrahedron Letters > 1998 > 39 > 27 > 4917-4920

The synthesis of covalent dimers of vancomycin which are linked from C- to N-terminus (head-to-tail linkage) is described. Such dimers have the potential to exploit additional cooperative interactions when binding to bacterial cell-wall precursors at a surface.

Publication date

Set your own date range

INFONA - science communication portal

Search results for: Dudley H. Williams

Enzyme catalysis from improved packing in their transition-state structures

Ligand Binding Energy and Enzyme Efficiency from Reductions in Protein Dynamics

Importance of Structural Tightening, as Opposed to Partially Bound States, in the Determination of Chemical Shift Changes at Noncovalently Bonded Interfaces

Order Changes within Receptor Systems upon Ligand Binding: Receptor Tightening/Oligomerisation and the Interpretation of Binding Parameters

Fragmentation Characteristics of Neutral N-Linked Glycans Using a MALDI-TOF/TOF Tandem Mass Spectrometer

Noncovalent Interactions: Defining Cooperativity. Ligand Binding Aided by Reduced Dynamic Behavior of Receptors. Binding of Bacterial Cell Wall Analogues to Ristocetin A

Ligand Binding Energy and Catalytic Efficiency from Improved Packing within Receptors and Enzymes

Isolation and Structure Elucidation of Chlorofusin, a Novel p53-MDM2 Antagonist from a Fusarium sp. [ J. Am. Chem. Soc. 2001 , 123 , 554−560].

Cooperative Binding Interactions of Glycopeptide Antibiotics

On the biosynthesis of an inhibitor of the p53/MDM2 interaction

An Enthalpic Component in Cooperativity: The Relationship between Enthalpy, Entropy, and Noncovalent Structure in Weak Associations

Enthalpy/Entropy Compensation as a Competition between Dynamics and Bonding: The Relevance to Melting of Crystals and Biological Aggregates

Isolation and Structure Elucidation of Chlorofusin, a Novel p53-MDM2 Antagonist from a Fusarium sp.

Binding of vancomycin group antibiotics to d-alanine and d-lactate presenting self-assembled monolayers

A Vesicle Capture Sensor Chip for Kinetic Analysis of Interactions with Membrane-Bound Receptors

Binding of glycopeptide antibiotics to a model of a vancomycin-resistant bacterium

Kinetic Analysis of Antibody-Antigen Interactions at a Supported Lipid Monolayer

Estimating binding constants – The hydrophobic effect and cooperativity

Surface plasmon resonance analysis at a supported lipid monolayer

Synthesis of Covalent Head-to-Tail Dimers of Vancomycin

Filter options

Publication date

Content availability

Keywords

Data set

Journal

INFONA - science communication portal

Search results for: Dudley H. Williams

Add recipient

Sending message cancelled

Are you sure you want to cancel sending this message?

Send message

Filter options

Publication date

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

Content availability

Keywords

Data set

Journal

Reporting an error / abuse

Sending the report failed

Accessibility options