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By whole cell transformation, 32 out of 71 strains showed OYEs activity toward maleimide in the first round screening. Among them, a Bacillus strain was identified to be active toward a selection of substrates with different electron-withdrawing groups. Two OYE homologous genes, bac-oye1 and bac-oye2 were cloned from this strain and overexpressed in Escherichia coli BL21(DE3). The recombinant enzyme...
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