The information and communication industry (ICT) has become more significant in the regional economic structure in the last two decades, having a notable impact on other industries. Although young in age, the number of firms or employees in the industry is considerable. Bathelt, Malmberg and Maskell (2004) and Trippl et al. (2009) have highlighted two different knowledge interactions within the ICT sector-local (i.e. local buzz) and trans-local (i.e. global pipelines). Furthermore, this knowledge interaction pattern can be different according to the size, economic structure and history of the cities/regions under consideration. The article is based on empirical research, focusing on the ICT sector knowledge interactions in the region of Eastern Slovakia. It highlights the pre-existing industry related basis of ICT, the importance of local and trans-local interactions, its social capital level as well as clustering potential.