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Simultaneous saccharification and fermentation (SSF) has been considered a promising and economical process for cellulosic ethanol production. Further cost savings could be gained by reducing enzyme loading and engineering host strain for ethanol production. In this study, we demonstrate efficient ethanol production by SSF without supplementation of β-glucosidase using an engineered Saccharomyces...
Functional expression of a cellodextrin transporter and an intracellular β-glucosidase from Neurospora crassa in Saccharomyces cerevisiae enables simultaneous co-fermentation of cellobiose and non-glucose sugars such as xylose. Here we investigate the functional role of aldose 1-epimerase (AEP) in engineered cellobiose utilization. One AEP (Gal10) and two putative AEPs (Yhr210c and Ynr071c sharing...
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