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To evaluate the impact of age on the expression of apoptotic biomarkers in human spermatozoa.Cross sectional, prospective study.Academic centers.Healthy volunteers with proven fertility, stratified by age (n = 25, range: 20–68 years).Examination of serum hormone levels and basic semen parameters, and assessment of early (plasma membrane translocation of phosphatidylserine) and late (DNA fragmentation)...
To determine the effect of semen storage and separation techniques on sperm DNA fragmentation.Controlled clinical study.An assisted reproductive technology laboratory.Thirty normoozospermic semen samples obtained from patients undergoing infertility evaluation.One aliquot from each sample was immediately prepared (control) for the sperm chromatin dispersion assay (SCD). Aliquots used to assess storage...
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