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The purpose of this study is to report successful pregnancies and deliveries from embryos derived from vitrified-warmed oocytes injected with frozen-thawed testicular sperm. After the vitrification-warming procedure, surviving oocytes from 11 patients were microinjected with thawed testicular spermatozoa, and subsequent viable cleaving embryos were transferred, resulting in four clinical pregnancies.
To assess the effects of vitrification on the biomolecular profile of oocytes, we analyzed through real-time reverse transcriptase–polymerase chain reaction eight genes encoding critically important proteins for embryo development and compared this partial transcriptome with that of freshly collected gametes isolated from the same women. The comparison of the molecular profiles demonstrated that our...
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