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The relative weight of the slowly decaying M intermediate of the photocycle of bacteriorhodopsin increases upon increasing the energy density of the short (10 ns) actinic laser pulse. Moreover, when a pre-exciting flash is applied to the BR sample, the absolute amplitude of the M s is higher in the signal induced by a second flash, applied with a delay from 100 μs to 100 ms. These facts together...
The kinetics of photoelectric and optical signals were measured on samples containing oriented purple membranes immobilized in a poly(vinyl alcohol) film and on purple membranes introduced into a PVA-H 2 O mixture. The bacteriorhodopsin photocycle in the PVA-H 2 O mixture was complete. The only observed changes were the slowing down of the optical and electrical signals in relation...
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