To compare muscle recruitment, maximal force, and rate of force development changes following different resistance exercise protocols with a constant volume-load.Within-subjects randomized crossover trial.Fourteen (n=14) resistance trained male participants completed three different resistance exercise protocols involving 20 squat repetitions, prescribed at 80% of 1-repetition-maximum. Protocol A consisted of 5 sets of 4 repetitions with 3min inter-set rest intervals, protocol B was 5 sets of 4 repetitions with 20s inter-set rest intervals, and the rest-pause method was an initial set to failure with subsequent sets performed with a 20s inter-set rest interval. Maximal squat isometric force output and rate of force development (RFD) were measured before, immediately upon completion (IP), and 5min (5P) following each protocol. Muscle activity from 6 different thigh and hip muscles was measured with surface electromyography (EMG) at each time point, and during every squat repetition.Participants completed the rest-pause method in 2.1±0.4 sets, with a total protocol duration of 103s compared to 140s and 780s for protocols B and A, respectively. All protocols elicited similar decreases (p<0.05) in maximal force and RFD at IP, with full recovery at 5P. Increased motor unit recruitment was observed during the rest-pause method compared to both protocols A and B for all muscles measured (p<0.05).As a result of the increased EMG during exercise and no greater post-exercise fatigue, it was concluded that the rest-pause method may be an efficacious training method for resistance-trained individuals.