To determine whether tester experience influences the reliability of three-dimensional gait collections.Reliability study.Ten healthy subjects visited a university gait laboratory on two separate days and underwent a walking gait analysis. During each visit, kinematic data were collected by a biomechanist with 8 years of 3D gait analysis experience (EXP) and a physical therapist with no previous 3D gait analysis experience (NOV).Joint kinematic angles were calculated using either a functional or predictive joint identification method. Within-tester and between-tester measures of reliability were determined by calculating the root mean square error (RMS) and coefficient of multiple correlations (CMC).Within-tester RMS and CMC values were not significantly different (P > 0.05) between the EXP and NOV testers using either a functional or predictive joint approach. Within-tester CMC values exceeded 0.90 for both testers across all kinematic variables. Between-tester CMC reliability values were greater than 0.85 for all variables measured.Following basic training, a physiotherapy clinician with no previous 3D gait experience is as reliable as an experienced gait biomechanist with respect to marker placement accuracy. In addition, reliability comparisons between an experienced and novice tester appear independent of the joint identification method chosen.