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The equilibrium kinetics of biomolecules can be probed by techniques such as temperature-jump or fluorescence correlation spectroscopy. These measurements can be described by dynamical fingerprints, i.e., densities of relaxation timescales where each peak corresponds to an exponential relaxation process. In many cases, single- or double-peaked fingerprints are found, suggesting that a two- or three-state...
Fluorescence correlation spectroscopy (FCS) measures the fluorescence fluctuations of fluorophores in solution, but is restricted to extremely low concentrations. Plasmonic gold bowtie nanoantennas enhance a single molecule’s fluorescence relative to a large background of unenhanced molecules, and here we show that bowties can extend FCS measurements to much higher concentrations. In this demonstration,...
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