Recent public health recommendations for step frequency over a given timeframe (stepsmin −1 ) associated with moderate-intensity physical activity (MPA) have been developed. The recommendation suggests 100 stepsmin −1 . This estimate overlooks the impact of anthropometric differences between individuals, notably leg length, which is related to step frequency. Therefore this study examined the impact of leg length on stepsmin −1 associated with MPA. Twenty adults age 20–40 years (age 26.4±4.6 years, 9 males) walked over-ground at five walking speeds (0.5ms −1 , 0.75ms −1 , 1.0ms −1 , 1.25ms −1 , and 1.5ms −1 ), lasting 6min each, while wearing a portable gas analyser. Participants’ step frequency (stepsmin −1 ) for each walking speed was determined using a hand-tally counter. Random effects models were used to predict stepsmin −1 from METs and participant anthropometric measures (body mass index and leg length [cm]). Model estimates were used to predict stepsmin −1 corresponding to heights ranging from 5ft. to 6ft. 6in. (6 in increments). Overall, 100 stepsmin −1 corresponded to expending 3 METs (SEE 3.49 stepsmin −1 , R 2 =0.68). As leg length increased estimated stepsmin −1 decreased by −1.15stepsmin −1 (95CI −2.19 to −0.10 stepsmin −1 ). Based on leg length for individuals 5ft to 6ft 6in., stepsmin −1 ranged from 111 to 85, respectively. Established stepsmin −1 cutpoints associated with MPA are general public health guidelines and anthropometric differences in leg length should be accounted for when developing step frequency recommendations for physical activity or weight loss studies that include individuals of varying height.