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In order to reduce both acquisition and reconstruction times, illumination and detection in fluorescence molecular tomography have recently evolved from a point-based to a pattern-based approach. However, the choice of the best set of source patterns to project onto the sample is still an open problem. Here, we introduce a novel method, namely the virtual source patterns method, which allows for considering...
Functional near infrared spectroscopy (fNIRS) is a non-invasive imaging modality to measure functional brain activities. Many researches have investigated diffuse optical tomography (DOT) to overcome the limitation of lack of depth information in fNIRS topographic approach. In this paper, we proposes a novel compressed sensing approach, especially using a 2-thresholding algorithm, that directly reconstructs...
This paper is an extension of our ISBI 2011 work, where we proposed a novel variational approach to estimate dense myocardial velocity fields from 4D ultrasound (4DUS). In this paper, we present a thorough performance evaluation of our method by comparing it with the primary method of record, which relies on cross-correlation. Our comparative performance evaluation uses several data sources, including...
Live cell microscopy images enable to study the motion of subcellular particles for understanding biological processes within cells. For accurate quantification of the particle motion, compensation of the motion and deformation of the cell nucleus is required. We introduce a multi-frame non-rigid registration approach for live cell microscopy image sequences. Compared to existing approaches using...
Diffuse optical imaging has been developed at University College London (UCL) as a new method of assessing cerebral function and pathology in the newborn infant brain. The technique exploits the relative transparency of biological tissue to near-infrared light (700–1000nm) and the wavelength dependent absorption characteristics of hemoglobin, which vary with oxygenation. Methods have been developed...
In this paper, we propose a method to improve the automatic detection of the optic disc on fundus images. We have studied and implemented some of the optic disc detectors from concerning literature to organize them into an ensemble system. As a former work, we proposed an ensemble-based optic disc detection system, based on simple majority voting which already outperformed the individual detectors...
The presentation gives an introduction and overview of our recently developed Stochastic Optical Fluctuation Imaging microscopy technique. The theoretical foundations are given and the method is exemplified on several model systems. Its advantages and limitations are discussed, and many applications of SOFI in cell microscopy are presented.
Cardiac gated SPECT imaging suffers from several degrading factors including limited data counts, depth-dependent blur, attenuation, scatter, and motion blur. In recent work we demonstrated that use of temporal processing could lead to the most improvement in 4D reconstruction. In this work we investigate how motion estimation models can affect the reconstruction in 4D, and in particular, we examine...
Super-resolution localization microscopy methods such as PALM and STORM have been shown to provide imaging with resolutions up to a few tens of nanometers while using relatively simple setups. Biplane PALM has extended the PALM technique to three-dimensions, by simultaneously using two imaging planes, with different focal depths. A key aspect in achieving good axial localization results is the alignment...
Polarization Second Harmonic Generation enables to quantify some of the underlying molecular details of key biological structures within muscle, axons and collagen at suboptical scales. However, it is only available in dedicated labs. In this work we detail a procedure to adapt and calibrate a commercial multiphoton microscope (Leica TCS-SP5) to acquire PSHG images reliably. For this, two different...
The main function of the respiratory system is gas exchange. Since many disease or injury conditions can cause biomechanical or material property changes that can alter lung function, there is great interest in measuring regional lung function and regional mechanical changes. The mechanical properties of lung parenchymal tissue are both elastic and dissipative, as well as being highly nonlinear. A...
Elevated intracranial pressure (ICP) is often associated with traumatic brain injury and can cause substantial complications. Elevated ICP also causes dilation of the optic nerve sheath, which can be observed and quantitated using intraocular ultrasound. We report on the use of 3-D ultrasound and automated processing algorithms to reduce operator variability in the assessment of sheath diameter. Using...
Three dimensional motion estimation is an active research field in ultrasound imaging, motivated by the recent progress in 3D acquisition. Out-of-plane motion and pattern decorrelation induced by azimuthal displacement yield bias in 2D estimation methods. However, 3D estimation may easily become time-consuming because of the large amount of data. Authors proposed different ways to estimate 3D displacement,...
Optical multispectral imaging during open neurosurgery can provide functional information equivalent to fMRI as well as information about blood flow and oxygenation dynamics. To perform multispectral analysis images acquired sequentially under different illumination wavelengths must be coregistered such that information about the same surface region can be obtained throughout the imaging time period...
Detection of the optic cup, which is an excavation in the optic disc, is an essential step in glaucoma assessment. In digital fundus photography, which captures 2D images of the retina, identification of the optic cup can be challenging. Kinks are bendings of vessels as they traverse the optic cup boundary, and are used clinically to determine the location of the optic cup. In this paper, we propose...
Primary ciliary dyskinesia (PCD) implies cilia with dysmotility or total absence of motility, which may result in sinusitis, chronicbronchitis, bronchiectasis and male infertility. A large number of deficiencies detectable on the ultrastructural level give rise to PCD, but patients with normal cilia ultrastructure are common. An early diagnosis is very important since PCD can cause permanent lung...
In this simulation study, an engineered point-spread function (PSF) with reduced depth variability (due to depth-induced aberrations) was applied to three-dimensional computational optical-sectioning microscopy (COSM) imaging to investigate its impact on image restoration. Intermediate synthetic images from PSF-engineered COSM in the presence of aberrations were computed and then processed using a...
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