Serine proteases are a family of enzymes that degrade a wide range of proteins and play vital roles in a variety of biological processes. Their mechanism of action involves several catalytic strategies that are common in enzymatic catalysis. In this article, we review the catalytic mechanism of serine proteases and how it was elucidated over the years. The artful application of chemical probes to dissect the mechanism of serine proteases provides excellent examples for teaching present-day students and scientists how to solve complex chemical and biological problems through chemical logic.
Graphic abstract