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The protein adsorption is a crucial but complex phenomenon in many applications, like biosensors, biomaterials. The amount, orientation and biological activity of adsorbed proteins are heavily dependent upon the structure of protein, like widely-applied Bovine Serum Albumin (BSA), and the properties of the substrate. At some extent, to explore the physicochemical properties and dynamics interaction...
The self-assembly adsorption of bovine serum albumin (BSA) on the hybrid surface including the discontinuous gold nano film and silica under liquid condition was studied in situ by atomic force microscope (AFM) at room temperature. To investigate the adsorption of BSA on silica and gold nano film simultaneously, the surface with discontinuous gold nano film and visible blank block on silica by physical...
The high molecular mass respiratory protein of the tarantula Eurypelma californicum, a 4×6-mer hemocyanin, was investigated by atomic force microscopy (AFM). Various substrates and methods were evaluated for immobilization of individual hemocyanin molecules on a solid surface. Samples were imaged after physisorption on mica and self-assembled monolayers, and after chemisorption on Au(111) and N-hydroxy-succinimide...
Nanostructures of lysozyme molecules and lysozyme-nanogold conjugates were generated by atomic force microscopy in contact-, tapping- and force-distance-mode on mica in aqueous solution. In contact mode at high ionic strength, adjusted lysozyme concentration and lower loading force a monolayer of defined structure and orientation of lysozyme can be formed by the scan process of the tip. A lateral...
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