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A set of shuttle vectors was constructed to facilitate expression of genes for metabolic engineering in Saccharomyces cerevisiae. Selectable markers include the URA3, TRP1, MET15, LEU2‐d8, HIS3 and CAN1 genes. Differential expression of genes can be achieved as each marker is available on both CEN/ARS‐ and 2 µ‐containing plasmids. Unique restriction sites downstream of TEF1, PGK1 or HXT7‐391 promoters...
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