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LKB1(Liver Kinase B1) gene is a tumor suppressor gene, which regulates the pathophysiological function of many cells, but the mechanism of LKB1 gene is still unclear. The double transgenic mice with positive homozygous and negative homozygous were screened by PCR amplification technique and gel electrophoresis technique. The expression level of LKB1 gene in smooth muscle cells was detected by Westernblot...
We investigate the anticancer effect of a traditional Chinese medicine gambogic acid (GA) in human gastric cancer line SGC-7901 and further study the mechanism of apoptosis induction of GA.Low differential human gastric cancer line SGC-7901 were treated with GA at different doses and different times, the inhibitory rates were detected by MTT assay. Apoptosis induced by GA in SGC-7901 cells was observed...
To explore the chronic hematologic toxicity in BALB/C mice exposed to low concentration of benzene which is equal to concentration of Chinese occupational hygiene standard, the benzene (20mg/m3) was applied to BALB/C mice by inhalation exposure, once per day, for 3 month and the mice were raised for another 90 days after exposure. During the experimental period, micronucleus frequency in peripheral...
Previous reports reveal that Isoliquiritigen(ISL), a licorice flavonoid, possesses various antitumour properties, cyclophosphamide, an alkylating agent, has various genotoxic and carcinogenic effects, and to be involved in some secondary neoplasmas. However, it is still used extensively as an antitumour agent and immunosuppressant in the clinic. In order to find out whether isoliquiritigen could enhance...
SET and MYND domain-containing protein 3 (SMYD3) is a novel histone methyltransferase involved in human carcinogenesis. To further establish the transgenic mice of SMYD3 and provide a useful platform for the study of SMYD3 and the screening of novel antitumor drugs, a transgene for liver-specific simultaneous expression of SMYD3 and EGFP was constructed. The human SMYD3 gene was subcloned into pIRES2-EGFP...
In order to explore potential damage effect of gaseous formaldehyde on genetic material of liver cells of mice. SPF-class Kun Ming male mice were used in experiment. The mice were exposed to gaseous formaldehyde in such concentration as 0 mg/m3, 0.5 mg/m3 and 3.0 mg/m3, the agent was administrated by continuous dynamic inhalation for 72 hours. After finished exposure to formaldehyde, observed the...
AIM: In the present study, murine A549 Lung cancer cells were provided Lung cancer with different thermal dose invitro and invivo, thereafter we investigated the apoptosis, necrosis rates, and intratumoral microvessel density (MVD) to determine that microvessel damage plays an important role in the tumoricidal effect of Lung cancer. Methods: A549 Lung cancer cells were inoculated in the right hind...
Antigenic differences between normal and malignant cells form the basis of clinical immunotherapy protocols. Because the antigenic phenotype varies widely among different cells within the same tumor mass, immunization with a vaccine that stimulates immunity to a broad array of tumor antigens expressed by the entire population of malignant cells is likely to be more efficacious than immunization with...
We investigated the feasibility of transferring naked plasmid DNA containing a therapeutic gene (IL-12) into mice harboring growing Renca tumors. We found that naked DNA transferred into growing Renca and B16(F10) tumors gives higher expression level of reporter gene than complexes of DNA with DDAB/ DOPE or DC-Chol/DOPE. Transfer of naked DNA carrying the IL-12 gene into growing Renca tumors causes...
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