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Patients infected human papillomavirus (HPV) is increasing all over the world, in particular the community of woman. Microarray-in-a-tube system is a novel method of integrating polymerase chain reaction (PCR) with the microarray technique and a simple and high-throughput detection system. In this work, the microarray-in-a-tube system used in the function of detection human papillomavirus genotypes...
Systematic evolution of ligands by exponential enrichment (SELEX) is a screening technique that involves a progressive selection of highly specific ligands by performing repeated rounds of partition and amplification from a large combinatorial nucleic acid library. The products of the selection are usually called aptamers, which are short single-stranded deoxyribonucleic acid (ssDNA) or ribonucleic...
Mitochondria DNA (mtDNA) plays an important role in both aging-associated degenerative diseases and cancers. In this study, a new microfluidic system integrating functional components including a mtDNA extraction module, a polymerase chain reaction (PCR) module and a micro-capillary electrophoresis (MCE) module has been developed. A new approach to vertically integrate these three modules has been...
Cancer is recognized as a serious health challenge both in the United States and throughout the world. While early detection and diagnosis of cancer leads to decreased mortality rates, current screening methods require significant time and costly equipment. Recently, increased levels of certain micro-ribonucleic acids (miRNAs) in the blood have been linked to the presence of cancer. While blood-based...
We performed on-chip DNA methylation analysis using methylation-specific PCR (MSP) within a high throughput microfluidic droplet array. The device uses of the oil phase as a companion fluid for both sample actuation and compartmentalization. These technical advantages allow for infusion of minute amounts of sample for arrayed MSP analysis, without the added complexities inherent in microfluidic droplet-based...
A multi-function, microfluidic module was designed, fabricated, and used for mutation detection using the ligase detection reaction (LDR) following amplification by the polymerase chain reaction (PCR). The module carried out four processing steps: (1) the PCR to amplify a target region in the K-ras gene of the human genome, which can lead to colorectal cancer; (2) the purification of the PCR products;...
In order to provide feasible primer set for performing a polymerase chain reaction (PCR) experiment, many primer design methods have been proposed. However, the majority of these methods require a long time to obtain an optimal solution since quantities of template DNA need to be analyzed, and the designed primer sets usually do not provide a specific PCR product size. Evolutionary computation has...
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