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Serotonin (5‐HT) production and expression of 5‐HT receptors (5‐HTRs) occur early during prenatal development. Recent evidence suggests that, in addition to its classical role as a neurotransmitter, 5‐HT regulates neuronal connectivity during mammalian development by modulating cell migration and neuronal cytoarchitecture. Given the variety of 5‐HTRs, researchers have had difficulty clarifying the...
A method is described to isolate hippocampal neurons from postnatal day 7 to postnatal day 21 rats for use in short term cultures using a combination of enzymatic and mechanical dissociation. A significant fraction of the cells (37.5±2.3%, n=10 cultures) were labelled with anti-GABA antibodies and the neurons survived a minimum of 8 days in culture. Patch-clamp recording in the current clamp mode...
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