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The subcellular localization and functional significance of neuronal nicotinic acetylcholine receptor α 4 -subunits were investigated in the rat hypothalamic supraoptic nucleus. A high level of α 4 mRNA expression was found in the magnocellular neurons in the supraoptic nucleus. Strong immunoreactitivy for α 4 in neurons of the supraoptic nucleus was detected in the rough endoplasmic...
Localization of nicotinic acetylcholine receptor (nAChR) α4 subunits was investigated in rat cerebral cortex using a monoclonal antibody against α4 subunits. The antibody depleted more than 70% of the [ 3 H]methylcarbamylcholine binding activity of the solubilized membrane fraction. By light microscopy α4-like immunoreactivity (α4-LI) was found through layers II to VI. The immunostaining...
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