Wyniki wyszukiwania dla: E. Darzynkiewicz
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Bioorganic & Medicinal Chemistry > 2013 > 21 > 24 > 7921-7928
Increased efficiency in splice-correction (splice-switching) has been shown by use of a synthetic RNA 5′-end nuclear localization signal composed of an m 3 G-CAP. Use of the m 3 G-CAP as an NLS signal for therapeutic compounds in vivo is likely to require additional stability towards enzymatic degradation. For this reason introduction of stabilizing modifications into the triphosphate...
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European Biophysics Journal > 2000 > 29 > 7 > 487-498
The kinetics of binding 7-methyl-GpppG, an analogue of the 5′-mRNA cap, to the cap-binding protein eIF4E, at 20 °C, in 50 mM Hepes-KOH buffer, pH 7.2, and 50, 150 and 350 mM KCl, was measured using a stopped-flow spectrofluorometer, and was simulated by means of a Brownian dynamics method. For most of the stopped-flow measurements a single bimolecular step is an inadequate description of the binding...
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