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Over the last two decades virus-like particles (VLPs) have become an important tool in biomedical research and medicine. VLPs are multi- protein structures that resemble viable virus particles in conformation but lack the viral genome. Consequently, they are non-infectious and non-replicative, but retain the ability to penetrate cells, making them useful for a vast spectrum of applications. Above...
A combination of culture-dependent and culture-independent approaches was employed to identify the bacterial diversity of Çamaltı solar saltern in Turkey. The bacterial communities of Çamaltı Saltern were analyzed by molecular techniques that included denaturing gradient gel electrophoresis of 16S rRNA gene fragments PCR amplified from DNA extracted from the water samples of the saltern and 16S rRNA...
Dibenzothiophene (DBT) is an organic sulfur compound which remains in oil after hydrodesulfurization (HDS) process and can be removed by biodesulfurization (BDS). A new strain of Paenibacillus validus (strain PD2) was isolated from oil contaminated soils that is able to desulfurize DBT. HPLC analysis and Gibb’s assay showed that this strain was capable to convert DBT to 2-Hydroxybiphenyl (2-HBP) as...
Human adenovirus (HAdV) diversity in sewage was assessed by species-specific molecular methods. Samples of raw sewage were collected in 14 sewage disposal systems from January to December 2011, in Poland. HAdVs were detected in 92.1% of the analysed sewage samples and was significantly higher at cities of over 100 000 inhabitants. HAdV DNA was detected in sewage during all seasons. The most abundant...
We investigated the effect of ciprofloxacin, rifampicine and doxycycline on myeloperoxidase (MPO) activity, glutathione (GSH) and malondialdehyde (MDA) levels in allergic asthma patients and healthy volunteers. Polymorphonuclear leukocytes(PMNs) were isolated with ficoll-hypaque gradient centrifugation method. MPO activity was assayed with modified o-dianisidine, GSH by Ellman’s and MDA levels by...
The aim of this study was to evaluate the potential diagnostic usefulness of the full-length recombinant Toxoplasma gondii MAG1 protein by determining the levels of specific IgM and IgG antibodies in mouse and human sera obtained from individuals with acute and chronic toxoplasmosis. The obtained results revealed that IgG antibodies against MAG1 are a sensitive and specific marker of T. gondii infection...
Scopulariopsis brevicaulis is known as the most common etiological factor of the mould toenail infections. There are also reports indicating that S. brevicaulis could cause organ and disseminated infections. Nowadays microscopic observations from the direct sample and culture are crucial for the appropriate recognition of the infection. In this paper a PCR-based method for S. brevicaulis detection...
This study was conducted to determine the prevalence of Campylobacter spp. isolated from dogs’ faecal samples. From June 2012 to June 2013, a total of 210 faecal samples from pet dogs living in different kennels (n=210) were collected by the owners in Greater Poland Voivodeship, Poznań District, Poland. The study revealed that 105 out of 210 faecal samples (50%) contained Campylobacter. The highest...
Background: Knowledge on HBV prevalence and genotype distribution in Europe still is hampered by lack of coherent data sampling, small numbers of patients studied so far, and also modern times migration which influences both parameters in a quite dynamic manner. To find out whether HBV prevalence and genotype distribution has undergone any significant changes over the past decades, we have analyzed...
Streptococcus anginosus (milleri) is a diverse group of gram positive bacteria. Molecular methods to establish relationship between strains are poorly developed. Therefore, main tool to study genetic variability is restriction fragment length polymorphism combined with pulsed field gel electrophoresis (RFLP-PFGE). In this communication, we present optimized protocol for S. anginosus PFGE analysis.
To more efficiently identify the microbial community of the yak rumen, the standardization of DNA extraction is key to ensure fidelity while studying environmental microbial communities. In this study, we systematically compared the efficiency of several extraction methods based on DNA yield, purity, and 16S rDNA sequencing to determine the optimal DNA extraction methods whose DNA products reflect...
In this study, we aimed to determine chemical composition and antibacterial activities of Satureja hortensis and Calamintha nepeta against to 20 phytopathogenic bacteria causing serious crop loss. The essential oils of S. hortensis and C. nepeta were isolated by the hydrodistillation method and the chemical composition of the essential oils were analyzed by GC-MS. The antibacterial properties of the...
Numerous studies have recently shown that molecular biology tools can allow for early diagnosis of pathogens and can substitute existing cost and time-taking traditional methods. One of them, the qPCR, is successfully used in microbiology and its utility has been assessed for many different biological materials. The aim of this study was to: 1) determine, optimize and apply qPCR as a method to detect...
The incidence rate of the infected and complex wound is established at approximately 40 000/1 million of the world’s adult population. The aim of this study was to assess the efficiency of three novel types of wound dressings comprising sodium chloride, metatitanic acid and silicon dioxide nanoparticles. The study design was to prove their antimicrobial properties against the microorganisms most commonly...
Loosening of the hip joint prosthesis is considered as one of the most significant postoperative complications in recent years. The laboratory diagnostic procedure used to differentiate periprosthetic infection from aseptic loosening is very difficult because of the biofilm which microorganisms form on the implant surface. The purpose of this research was to evaluate the level of concordance between...
The objective of this paper is to review and summarize the antimicrobial efficacy of the acyldepsipeptides and to indicate the prospects of the therapeutic values of these compounds. This work is enriched by the description of the mutations within the clpP1clpP2 and clpP3clpP4 operons of Streptomyces lividans, which are considered to be the potential mechanism of the acyldepsipeptide (ADEP) – resistance...
We assessed the effect of β-Glucan on macrophages by Griess reagent and viability by MTT assay and cytotoxicity. Assay of macrophages culture supernatants were carried out on WEHI-164 fibrosarcoma cell line as tumor necrosis factor-α bioassay were done. NO release was increased at the dose of 10 μg/ml (P=0.001) of β-Glucan while the viability of macrophages in all concentrations was the same. In TNF-α...
The presence of Gram-negative bacteria species, other than Salmonella spp., in the gallbladder of pigs was examined. Isolated Gram-negative bacteria were assigned to species using the Microgen™ GnA+B-ID Systems. Of the 64 isolated strains 43 were identified as Escherichia coli, seven as Enterobacter spp., three each as Klebsiella spp., Citrobacter freundii, Aeromonas hydrophila and Cronobacter sakazakii...
Intestinal sulfate-reducing bacteria reduce sulfate ions to hydrogen sulfide causing inflammatory bowel diseases of humans and animals. The bacteria consume lactate as electron donor which is oxidized to acetate via pyruvate in process of the dissimilatory sulfate reduction. Pyruvate-ferredoxin oxidoreductase activity and the kinetic properties of the enzyme from intestinal sulfate-reducing bacteria...
Yersinia secretion apparatus (Ysa), the chromosomal type three secretion system (T3SS) is considered to contribute to virulence of highpathogenicity Yersina enterocolitica biovar 1B. DNA-sequence of Ysa pathogenicity island was determined for clinical isolate DM0110 of Y. enterocolitica 1B/O8 with origin in Poland. We found a premature stop-codon in the regulatory gene ysrR (mutation at position 269)...
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