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The association between the presence of self-splicing intron Ca.LSU and proteolytic activity of Candida albicans isolates was tested. Study included 95 C. albicans strains isolated from gastrointestinal tract of diabetes children. The strains with the intron (genotype B) displayed a significantly higher proteolytic activity (385.2 ± 192U/L) than did strains without intron (genotype A) (119 ± 115U/L)...
Lactobacilli were isolated from chicken gastrointestinal tract and examined for their potentially probiotic properties towards their inhibitory activity against poultry enteropathogenic bacteria. Biochemical tests, ITS-PCR and cell wall protein analysis were used to characterize the Lactobacillus isolates. The identification of isolated Lactobacillus strains based on phenotypic properties was not...
Bacteria of Desulfovibrio desulfuricans species are Gram-negative, anaerobic rods selectively reducing sulphates and colonizing oxygen-free ecosystems. They are ubiquitous in the natural environment and have been also found to reside in the human digestive tract. They are suggested to be involved in the pathogenesis of ulcerative colitis and Crohn's disease. The D. desulfuricans wild strains were...
The probiotic potential of 3 yeasts strains of Saccharomyces cerevisiae isolated from kefirs and feces was investigated and compared with 3 isolates from medicines and 2 collection strains (ATCC) of Saccharomyces cerevisiae var. houlardii. Genetic identification of yeasts based on karyotypes indicated their affiliation to Saccharomyces spp. although chromosomal polymorphism was observed. Concerning...
Enterococcus faecalis represents recently an important etiological agent of health care-associated infections (HAIs) and there is a need for evaluation and comparison of typing methods available for this microorganism. We tested multilocus VNTR (variable-number tandem repeats) analysis (MLVA) on a well-characterized collection of 153 clinical isolates of E. faecalis, corresponding to 52 multilocus...
Gut microbiota was analyzed in children, aged 6–18 months and su$ering from atopic dermatitis before and after 3 month supplementation of their diet with Lactobacillus casei DN – 114001 in a dose of 10⁹ cells daily. On completion of this period the total number of fecal Lactobacillus sp. cells decreased from 7.86 Log₁₀ CFU/g to 6.40 Log₁₀ CFU/g. After the next 5 months (without dietary supplementation...
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