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PCR-RFLP analysis of commonly occurring insertion sequences ISS1-type, IS904 and IS982 in Lactococcus sp. and Leuconostoc sp. was used for the genetic differentiation of 17 strains of lactic acid bacteria. ISS1-type and IS982 were found in all analysed strains while 1S904 was present exclusively in strains belonging to Lactococcus sp. Amplification of ISS1-type IS sequences resulted in formation of...
The aim of this study was to examine 29 strains of lactic acid bacteria of the Lactobacillus and Lactococcus genera, assessing their sensitivity to ochratoxin A and their ability to remove it from a liquid medium. It was demonstrated that most strains are insensitive to the presence of OTA at the quantity of 5 μg/disc. It was demonstrated that all strains caused a reduction of the toxin amount in...
Response surface methodology was used to optimize media components such as carbon and nitrogen (simple and complex) sources, mineral agents and growth factors (B vitamins, amino acids) for enhancing the biomass production of Lactobacillus rhanmosus PEN. For screening experiment the following carbon sources were selected: glucose, glucose+pyruvate, glucose+citrate, glucose+lactate, galactose, fructose,...
Four lactobacilli strains (Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacilus casei and Lactobacillus reuteri) were grown in MRS broth and three lactococci strains (Streptococcus thermophilus, Lactococcus lactis subsp. Lactis and Lactococcus lactis subsp. lactis biovar. diacetilactis) were grown in M17 broth. L. reuteri and S. thermophilus were chosen on the basis of the best mean β-galactosidase...
Lactobacillus rhamnosus E/N is a probiotic bacterium, which synthesizes exopolysaccharides (EPS) with significant bifidogenic and antioxidant activities. The sugar composition of the EPSs produced depended on carbohydrates used as a carbon source in the growth media. Five Bifidobacterium strains were tested in vitro for their ability to utilize all the EPSs studied. The highest bifidogenic activity...
The aim of this study was to identify various bacteria isolated from grapes and their wines. Additionally we investigated the capacity of lactic acid bacteria for microbiological deacidification of wines produced in Poland. We have identified Oenococcus oeni, Lactobacillus acidophilus and Lactobacillus delbrueckii. During the microbial deacidification process, we observed decreases of total acidity...
Bacteriocines are small peptides with anti-bacterial properties. They are produced both by Gram-positive and Gram-negative bacteria. Until now, a few hundred bacteriocines were described. Classification of bacteriocines undergoes continuous alterations, as new developments regarding their structure, amino acid sequence and recognised mechanism of their action are available. Some of bacteriocins (lantibiotics)...
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