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In this study, favorable carbon-nitrogen ratio for high yields of gibberellic acid (GA₃) production from Pseudomonas sp. was investigated. First of all, optimum carbon (glucose, maltose, sucrose, fructose, lactose) and nitrogen (KNO₃, NH₄C1, NaNO₃, urea, glycine) sources among the others were chosen. The highest yield of GA₃ productivity was found in growth medium supplemented with fructose (168.5...
Changes in cellular fatty acid composition during naphthalene degradation, at the concentrations of 0.5 g l⁻¹ or 1.0 g l⁻¹, by Pseudomonas sp. JS150 were investigated. In response to naphthalene exposure an increase in saturated/unsaturated ratio was observed. Additionally, the dynamic changes involved alterations in the contents of hydroxy, cyclopropane and branched fatty acids. Among the classes...
Studies were carried out on the microflora of phyllosphere and soil rhizosphere of hulled (Chwat variety) and naked (Akt variety) oats. The material taken for study embraced samples of leaves and soil rhizosphere taken from cultivations differing in extent of nitrogen fertilization. The studies involved determination of total number of aerobic heterotrophic bacteria belonging to the genus Pseudomonas...
Some parts of the Indian Himalayan region are covered by established and abandoned tea bushes. Rhizospheric soils of these plants were studied for bacterial dominance and antagonism. Representatives of Bacillus and Pseudomonas genera were found to dominate the rhizosphere of established and abandoned tea bushes, respectively. Amongst the isolated species Bacillus subtilis and Bacillus mycoides appeared...
In this work, we present the construction of a metagenomic library in Escherichia coli using pUC19 vector and environmental DNA directly isolated from Antarctic topsoil and screened for lipolytic enzymes. Screening on agar supplemented with olive oil and rhodamine B revealed one clone with lipolytic activity (Lip 1) out of 11000 E. coli clones. This clone harbored a plasmid, pLip 1, which has an insert...
Nickel resistant bacteria (ZB, ZC, ZD, ZL, ZK and SIX) were isolated from industrial effluents and corroded iron pieces from indigenous environment of Punjab, Pakistan. These six strains could tolerate nickel at different levels with ZB, ZC, ZD, ZL, ZK, and SIX having 233, 225, 267,233,228 and 296 mM minimum inhibitory concentration (MIC) of nickel ions, respectively. These bacteria were sensitive...
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