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Among 73 clinical isolates of Pseudomonas aeruginosa 48 strains were Ciprofloxacine (CIP) susceptible and 25 CIP resistant (Minimal inhibitory concentration - MIC>32 μg/ml - 14 strains) or of intermediate susceptibility to CIP (MIC≥ 1,5-32 μg/ml - 11 isolates). Mutations in the quinolone-resistance-determining region (QRDR) of gyrA gene were searched in groups of CIP resistant and of intermediate...
Resistance causes of moulds to N,N-bis(3-aminopropyl)dodecylamine (APDA) for selected species of Aspergillus niger and Aspergillus flavus was examined. Control (sensitive) strains and resistant strains, cultured at 0.05% triamine, were used in the experiments. The non-resistant strains did not have growth capacity in this amount of ADPA while the resistant strains were characterised by a smaller biomass...
A number of Enterococcus strains with high-level inducible resistance to vancomycin have been identified, and the relative incidence of these strains has increased significantly in the last years. The first outbreak caused by vancomycin-resistant enterococci in Poland was reported in 1999. Vancomycin-resistant Enterococcus faecium is known for its propensity to cause infections which are difficult...
The activity of ciprofloxacin, sparfloxacin and moxifloxacin was determined for 205 Enterococcus faecalis isolates from patients of five hospitals (Warsaw, Poland; collected from 2000 to 2002). Ciprofloxacin resistant and intermediate isolates were numerous (53.7%). Among them, highly resistant (MIC≥16 mg/l) isolates predominated (98%). Isolates resistant to ciprofloxacin were also resistant to sparfloxacin...
This study analysed the pattern of antibiotic resistance in 251 Campylobacter strains isolated from symptomatic children hospitalized in 4 large paediatric hospitals in Poland from 2000 through 2007. The highest resistance was found for ciprofloxacin (49.5% for C. jejuni and 51.3% for C. coli), followed by tetracycline (17.5% and 18.0%, respectively), and ampicillin (13.2% and 10.2%, respectively)...
The natural ability of microorganisms for adhesion and biofilm formation on various surfaces is one of the factors causing the inefficiency of a disinfection agent, despite its proven activity in vitro. The aim of the study was to determine the effectiveness of disinfecting substances on bacterial biofilms formed on stainless steel surface. A universally applied disinfecting agent was used in the...
Meticillin-resistant Staphylococcus aureus (MRSA) is one of the main causes of hospital-acquired infections, but since late 1990s also the community-acquired. For better understanding of the S. aureus epidemiology there is an urgent need for creation of new typing method for SCCmec element. The molecular typing of MRSA for epidemiological purposes is investigated by pulsed-field gel electrophoresis...
Enterococcus faecalis plays a significant role in hospital-acquired infections (HAIs), and combination of penicillin with aminoglycoside is important in therapy of invasive HAIs. Penicillin resistance in this organism is due to modification of the drug target, penicillin-binding protein (PBP5), its overproduction and expression of β-lactamase. Although rare, this phenotype is often associated with...
Nickel resistant bacteria (ZB, ZC, ZD, ZL, ZK and SIX) were isolated from industrial effluents and corroded iron pieces from indigenous environment of Punjab, Pakistan. These six strains could tolerate nickel at different levels with ZB, ZC, ZD, ZL, ZK, and SIX having 233, 225, 267,233,228 and 296 mM minimum inhibitory concentration (MIC) of nickel ions, respectively. These bacteria were sensitive...
The resistance of microorganisms to antibiotics has become a serious issue in recent years in the therapy of bacterial infections. This problem also concerns the treatment of infections caused by Helicobacter pylori strains. The aim of this study was to evaluate the frequency of primary resistance of H. pylori strains isolated from children and adults. The subject of the research was 105 strains of...
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