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The study included a sheep flock comprising five genetic groups. The ELISA was applied to perform constant monitoring (every six months) for the infection of ewes with small ruminant lentivirus (SRLV). The research results demonstrated a negative effect of SRLVs infection on lamb rearing that, depending on the genetic group, proved to be lower 1.3%-1.4% compared to the seronegative mothers. At relatively...
In the study, a 122 bp fragment of gag gene encoding immunodominant epitope on capsid protein of small ruminant lentiviruses (SRLVs) found in sheep was amplified by PCR and analysed by SSCP and sequencing. Out of 30 DNA samples, five showed different migration patterns, demonstrating the individual variations within gag sequences, which were confirmed afterwards by sequence analysis. In two samples...
A seroprevalence study of bovine immunodeficiency virus (BIV) was undertaken on 1,541 serum samples from Holstein cattle from 23 herds, located in different geographical regions of Poland. The analysis was performed using ELISA, with recombinant Gag protein of BIV as antigen. The average BIV prevalence was 4.9% in individual cattle, while the percentage of herds harboring at least one seropositive...
Samples of morbid bovine tissues obtained from slaughterhouse were submitted to histopathological examination. Neoplastic growth within the peripheral nerves was observed microscopically, with the presence of Antoni A and Antoni B patterns, and sporadically Verocay bodies. Additionally, immunohistochemical examination of the sections revealed positive reaction to vimentine, neuron specific enolase,...
The aim of these studies was to determine the performances of five commercial ELISA kits in the detection of BLV antibodies in serum samples. Different variants of ELI SA methods (indirect, blocking and double-well) were employed for these kits. Comparative investigations using 428 serum samples showed 418 consistent results (97.7%). Ten results (2.3%) proved to be inconsistent including 7 (1.6%)...
The aim of this study was to determine the titre of IgG₁, IgG₂, and lgM antibodies against bovine leukaemia virus (BLV) in the serum of either experimentally or naturally infected animals. Experimental infection performed in calves injected intravenously with blood from leukaemic cattle. The presence of BLV antibodies belonging to lgG₁, IgG₂, and IgM classes were studied for 7 months using the ELISA...
The aim of the experiments conducted was to modify the indirect ELISA to determine different immunoglobulin classes in the reaction of antibodies with bovine leukaemia virus. Rabbit sera immunized against bovine IgG₁, IgG₂ and IgM as well as serum conjugates were used in the modified method. Examinations of 100 reference serum samples from BLV-infected animals and 50 samples from BLV-free animals...
The influence of time and storage conditions on the stability of specific antibodies against EBLV was determined. The titer of antibodies was tested by use of the immunodiffusion test and ELISA. The sera in the lyophilized form with the addition of 0.01% sodium azide and 0.01% merthiolate were stored at + 4°C, — 20°C and at — 20°C after freezing and thawing. Sera with the above preparations stored...
Appearance of IgG₁, IgG₂, IgM in blood serum was investigated in 12 calves, experimentally inoculated with BLV, immediately after birth. The titer of BLV antibodies, was also examined by means of ID and ELISA tests. An increase of IgG₂ fractions has been found in the first week after infection. However, this protein seems not to be a specific antibody. Properties of antibody have been revealed by...
Polish ELISA set, prepared in the Veterinary Research Institute, has been compared with sets produced in the German Federal Republic, Czechoslovakia, France and Belgium. Comparative examinations were performed in a herd comprising 44 cows which revealed a high percentage of positive reagents in the AGID test. In general, the results obtained by means of different tests, recommending high dilution...
Pure IgG₁ and IgG₂ globulins were obtained by means of precipitation with ammonium sulphate and subsequent fractionation in DEAE Sephadex A-50 column. The IgM globulin was isolated by means of chromatography on Sephadex G-200 and DEAE-cellulose. Antibodies to these proteins were produced by immunization of rabbits and goats. Purity of proteins and monovalence of antibodies were checked up by means...
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