Zhongsheng You

article

Control of gene expression through the nonsense-mediated RNA decay pathway

Andrew Nickless, Julie M. Bailis, Zhongsheng You

Cell & Bioscience > 2017 > 7 > 1 > 1-12

Nonsense-mediated RNA decay (NMD) was originally discovered as a cellular surveillance pathway that safeguards the quality of mRNA transcripts in eukaryotic cells. In its canonical function, NMD prevents translation of mutant mRNAs harboring premature termination codons (PTCs) by targeting them for degradation. However, recent studies have shown that NMD has a much broader role in gene expression...

article

Poly(ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities

Abigael Cheruiyot, Sharad C. Paudyal, In-Kwon Kim, Melanie Sparks, more

DNA Repair > 2015 > 35 > C > 106-115

Exonuclease 1 (Exo1) has important roles in DNA metabolic transactions that are essential for genome maintenance, telomere regulation and cancer suppression. However, the mechanisms for regulating Exo1 activity in these processes remain incompletely understood. Here, we report that Exo1 activity is regulated by a direct interaction with poly(ADP-ribose) (PAR), a prominent posttranslational modification...

article

DNA damage and decisions: CtIP coordinates DNA repair and cell cycle checkpoints

Zhongsheng You, Julie M. Bailis

Trends in Cell Biology > 2010 > 20 > 7 > 402-409

Maintenance of genome stability depends on efficient, accurate repair of DNA damage. DNA double-strand breaks (DSBs) are among the most lethal types of DNA damage, with the potential to cause mutation, chromosomal rearrangement, and genomic instability that could contribute to cancer. DSB damage can be repaired by various pathways including nonhomologous end-joining (NHEJ) and homologous recombination...

article

CtIP Links DNA Double-Strand Break Sensing to Resection

Zhongsheng You, Linda Z. Shi, Quan Zhu, Peng Wu, more

Molecular Cell > 2009 > 36 > 6 > 954-969

In response to DNA double-strand breaks (DSBs), cells sense the DNA lesions and then activate the protein kinase ATM. Subsequent DSB resection produces RPA-coated ssDNA that is essential for activation of the DNA damage checkpoint and DNA repair by homologous recombination (HR). However, the biochemical mechanism underlying the transition from DSB sensing to resection remains unclear. Using Xenopus...

article

The F Box Protein Fbx6 Regulates Chk1 Stability and Cellular Sensitivity to Replication Stress

You-Wei Zhang, John Brognard, Chris Coughlin, Zhongsheng You, more

Molecular Cell > 2009 > 35 > 4 > 442-453

ATR and Chk1 are two key protein kinases in the replication checkpoint. Activation of ATR-Chk1 has been extensively investigated, but checkpoint termination and replication fork restart are less well understood. Here, we report that DNA damage not only activates Chk1, but also exposes a degron-like region at the carboxyl terminus of Chk1 to an Fbx6-containing SCF (Skp1-Cul1-F box) E3 ligase, which...

article

Monitoring ATM kinase activity in living cells

Sam A. Johnson, Zhongsheng You, Tony Hunter

DNA Repair > 2007 > 6 > 9 > 1277-1284

DNA double strand breaks (DSB) in mammalian cells result in the activation of the ATM protein kinase. This leads to phosphorylation of numerous downstream transducer and effector proteins that coordinate a cellular response including DNA repair, cell cycle arrest or apoptosis. We have developed a reporter protein that allows the measurement of ATM kinase activity in single living cells. This CFP-YFP...

article

Xenopus ATR is a replication-dependent chromatin-binding protein required for the DNA replication checkpoint

Mohammad Hekmat-Nejad, Zhongsheng You, Muh-ching Yee, John W Newport, more

Current Biology > 2000 > 10 > 24 > 1565-1573

Background: The DNA replication checkpoint ensures that mitosis is not initiated before DNA synthesis is completed. Recent studies using Xenopus extracts have demonstrated that activation of the replication checkpoint and phosphorylation of the Chk1 kinase are dependent on RNA primer synthesis by DNA polymerase α, and it has been suggested that the ATR kinase—so-called because it is related to the...

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Search results for: Zhongsheng You

Control of gene expression through the nonsense-mediated RNA decay pathway

Poly(ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities

DNA damage and decisions: CtIP coordinates DNA repair and cell cycle checkpoints

CtIP Links DNA Double-Strand Break Sensing to Resection

The F Box Protein Fbx6 Regulates Chk1 Stability and Cellular Sensitivity to Replication Stress

Monitoring ATM kinase activity in living cells

Xenopus ATR is a replication-dependent chromatin-binding protein required for the DNA replication checkpoint

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INFONA - science communication portal

Search results for: Zhongsheng You

Control of gene expression through the nonsense-mediated RNA decay pathway

Poly(ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities

DNA damage and decisions: CtIP coordinates DNA repair and cell cycle checkpoints

CtIP Links DNA Double-Strand Break Sensing to Resection

The F Box Protein Fbx6 Regulates Chk1 Stability and Cellular Sensitivity to Replication Stress

Monitoring ATM kinase activity in living cells

Xenopus ATR is a replication-dependent chromatin-binding protein required for the DNA replication checkpoint

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