Search results for: Stig Uggerhøj Andersen

Items from 1 to 6 out of 6 results

article

Widespread and transgenerational retrotransposon activation in inter‐ and intraspecies recombinant inbred populations of Lotus japonicus

Eigo Fukai, Manabu Yoshikawa, Niraj Shah, Niels Sandal, more

The Plant Journal > 111 > 5 > 1397 - 1410

Transposable elements (TEs) constitute a large proportion of genomes of multicellular eukaryotes, including flowering plants. TEs are normally maintained in a silenced state and their transpositions rarely occur. Hybridization between distant species has been regarded as a ‘shock’ that stimulates genome reorganization, including TE mobilization. However, whether crosses between genetically close parents...

chapter

Vectors for reverse genetics and expression analysis

Stig Uggerhøj Andersen, Cristina Cvitanich, Mette Grønlund, Hanne Busk, more

Lotus japonicus Handbook > Transformation and Reverse Genetic Analysis > 289-292

The binary Agrobacterium pPZP211 vector is stable and fully sequenced. We have produced derivatives of this vector that can be used for general over-expression, nodule-specific expression, expression pattern analysis, and protein localization studies.

article

The deubiquitinating enzyme AMSH1 is required for rhizobial infection and nodule organogenesis in Lotus japonicus

Anna Małolepszy, Dorian Fabian Urbański, Euan K. James, Niels Sandal, more

The Plant Journal > 83 > 4 > 719 - 731

Legume–rhizobium symbiosis contributes large quantities of fixed nitrogen to both agricultural and natural ecosystems. This global impact and the selective interaction between rhizobia and legumes culminating in development of functional root nodules have prompted detailed studies of the underlying mechanisms. We performed a screen for aberrant nodulation phenotypes using the Lotus japonicus LORE1...

article

RNA-Seq analysis and annotation of a draft blueberry genome assembly identifies candidate genes involved in fruit ripening, biosynthesis of bioactive compounds, and stage-specific alternative splicing

Vikas Gupta, April D Estrada, Ivory Blakley, Rob Reid, more

GigaScience > 2015 > 4 > 1 > 1-22

Background Blueberries are a rich source of antioxidants and other beneficial compounds that can protect against disease. Identifying genes involved in synthesis of bioactive compounds could enable the breeding of berry varieties with enhanced health benefits. Results Toward this end, we annotated a previously sequenced draft blueberry genome assembly using RNA-Seq data from five...

article

Lotus japonicus SUNERGOS1 encodes a predicted subunit A of a DNA topoisomerase VI that is required for nodule differentiation and accommodation of rhizobial infection

Hwi Joong Yoon, Md Shakhawat Hossain, Mark Held, Hongwei Hou, more

The Plant Journal > 78 > 5 > 811 - 821

A symbiotic mutant of Lotus japonicus, called sunergos1‐1 (suner1‐1), originated from a har1‐1 suppressor screen. suner1‐1 supports epidermal infection by Mesorhizobium loti and initiates cell divisions for organogenesis of nodule primordia. However, these processes appear to be temporarily stalled early during symbiotic interaction, leading to a low nodule number phenotype. This defect is ephemeral...

article

Genome‐wide LORE1 retrotransposon mutagenesis and high‐throughput insertion detection in Lotus japonicus

Dorian Fabian Urbański, Anna Małolepszy, Jens Stougaard, Stig Uggerhøj Andersen

The Plant Journal > 69 > 4 > 731 - 741

Use of insertion mutants facilitates functional analysis of genes, but it has been difficult to identify a suitable mutagen and to establish large populations for reverse genetics in most plant species. The main challenge is developing efficient high‐throughput procedures for both mutagenesis and identification of insertion sites. To date, only floral‐dip T‐DNA transformation of Arabidopsis has produced...

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Search results for: Stig Uggerhøj Andersen

Widespread and transgenerational retrotransposon activation in inter‐ and intraspecies recombinant inbred populations of Lotus japonicus

Vectors for reverse genetics and expression analysis

The deubiquitinating enzyme AMSH1 is required for rhizobial infection and nodule organogenesis in Lotus japonicus

RNA-Seq analysis and annotation of a draft blueberry genome assembly identifies candidate genes involved in fruit ripening, biosynthesis of bioactive compounds, and stage-specific alternative splicing

Lotus japonicus SUNERGOS1 encodes a predicted subunit A of a DNA topoisomerase VI that is required for nodule differentiation and accommodation of rhizobial infection

Genome‐wide LORE1 retrotransposon mutagenesis and high‐throughput insertion detection in Lotus japonicus

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