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Background Novel microscopic techniques which bypass the resolution limit in light microscopy are becoming routinely established today. The higher spatial resolution of super-resolution microscopy techniques demands for precise correction of drift, spectral and spatial offset of images recorded at different axial planes. Methods We employ a hydrophilic gel matrix for super-resolution microscopy...
Super-resolution microscopy is a powerful tool for understanding cellular function. However one of the most important biomolecules – DNA – remains somewhat inaccessible because it cannot be effectively and appropriately labeled. Here, we demonstrate that robust and detailed super-resolution images of DNA can be produced by combining 5-ethynyl-2′-deoxyuridine (EdU) labeling using the ‘click chemistry’...
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