Search results for: Kunio Fujiwara

Items from 1 to 20 out of 26 results

article

High-resolution separation of neodymium and dysprosium ions utilizing extractant-impregnated graft-type particles

Shoichiro Uchiyama, Takaaki Sasaki, Ryo Ishihara, Kunio Fujiwara, more

Journal of Chromatography A > 2018 > 1533 > C > 10-16

An efficient method for rare metal recovery from environmental water and urban mines is in high demand. Toward rapid and high-resolution rare metal ion separation, a novel bis(2-ethylhexyl) phosphate (HDEHP)-impregnated graft-type particle as a filler for a chromatography column is proposed. To achieve rapid and high-resolution separation, a convection-flow-aided elution mode is required. The combination...

article

Expression and distribution patterns of spermine, spermidine, and putrescine in rat hair follicle

Yutaro Yamamoto, Takamitsu Makino, Hideo Kudo, Hironobu Ihn, more

Histochemistry and Cell Biology > 2018 > 149 > 2 > 161-167

No expression and distribution patterns of polyamines (PAs), spermine, spermidine, and their precursor putrescine in mammalian hair follicle are available, although polyamines are known to correlate well with hair growth and epidermal tumor genesis. Immunohistochemistry (IHC) using our original two monoclonal antibodies (mAbs) ASPM-29 specific for spermine or spermidine, and APUT-32 specific for putrescine...

article

A simple in situ preconcentration method for the simultaneous determination of major and minor alkali and alkaline-earth metals in natural waters using iminodiacetate-grafted fiber

Fyodor S. Kot, Kunio Fujiwara, Malik Kochva

Analytical and Bioanalytical Chemistry > 2017 > 409 > 16 > 3917-3922

A simple and effective procedure was developed for the simultaneous in situ preconcentration of major and minor alkali and alkaline-earth metals (Li, Na, K, Rb, Mg, Ca, Sr, and Ba) from natural waters with iminodiacetate-grafted fiber (IDA-fiber), using soil water extracts as an example. The method was verified on mixed standard solutions of the metals at concentrations and ionic strengths reported...

article

On boron turnover in plant–litter–soil system

Fyodor S. Kot, Ronit Farran, Kunio Fujiwara, Galina V. Kharitonova, more

Geoderma > 2016 > 268 > C > 139-146

Boron (B) is an indispensable constituent of plant lignin st\ructures inherited by soil humus compounds. However, little is known about plant litter as a factor of B turnover in the plant–soil system. Also, the sources of soil available B and the mechanism of its release and mobility are poorly understood. In a series of experiments, we considered (1) leachates from decomposing plant litter and leaves...

article

Role of polyamines at the G1/S boundary and G2/M phase of the cell cycle

Tomoko Yamashita, Kazuhiro Nishimura, Ryotaro Saiki, Hiroyuki Okudaira, more

International Journal of Biochemistry and Cell Biology > 2013 > 45 > 6 > 1042-1050

The role of polyamines at the G ₁ /S boundary and in the G ₂ /M phase of the cell cycle was studied using synchronized HeLa cells treated with thymidine or with thymidine and aphidicolin. Synchronized cells were cultured in the absence or presence of α-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase, plus ethylglyoxal bis(guanylhydrazone) (EGBG), an inhibitor...

article

Antimicrobial fabric adsorbed iodine produced by radiation-induced graft polymerization

Shoji Aoki, Kunio Fujiwara, Takanobu Sugo, Koichi Suzuki

Radiation Physics and Chemistry > 2013 > 84 > Complete > 242-245

Antimicrobial fabric was synthesized by radiation-induced graft polymerization of N-vinyl pyrrolidone onto polyolefine nonwoven fabric and subsequent adsorption of iodine. In response of the huge request for the antimicrobial material applied to face masks for swine flu in 2009, operation procedure of continuous radiation-induced graft polymerization apparatus was improved. The improved grafting production...

article

An in vivo role of Mrp2 in the rat hepatocytes by immunocytochemistry for amoxicillin using the transporter-deficient EHBR

Kunio Fujiwara, Masashi Shin, Yohei Yoshizaki, Tsubasa Miyazaki, more

Journal of Molecular Histology > 2012 > 43 > 3 > 371-378

An in vivo role of the multidrug resistant-associated protein (Mrp2) in rat hepatocytes was examined by immunocytochemistry (ICC) for amoxicillin (AMPC) by the use of the transporter-deficient Eisai hyperbilirubinemic rats (EHBR). The ICC revealed that in the liver of EHBR at 3-h post-administration, amoxicillin accumulated in the cytoplasmic pools and nuclei of the hepatocytes in a characteristic...

article

Distribution study of peplomycin in rat kidney revealed by immunocytochemistry using monoclonal antibodies

Kunio Fujiwara, Masashi Shin, David M. Hougaard, Tetsuya Saita

Histochemistry and Cell Biology > 2011 > 135 > 1 > 93-101

Peplomycin (PEP), an anti-tumor antibiotic related structurally to bleomycin, is widely used, especially for squamous cell carcinoma but shows renal toxicity. We prepared monoclonal antibodies (mAbs) against N-(γ-maleimidobutyryloxy)succinimide-conjugated PEP. The mAbs were monospecific for PEP, but did not react with bleomycin and other anticancer antibiotics. The mAbs enabled us to develop an immunocytochemical...

article

A probable relationship between characteristic accumulation of doxorubicin and P-glycoprotein transporter in rat liver

Kunio Fujiwara, Masashi Shin, Tsubasa Miyazaki

Journal of Molecular Histology > 2011 > 42 > 5 > 409-415

Correlations between the distribution of anthracycline antibiotics doxorubicin (DX) and daunorubicin (DR) in the liver of rats injected with a single i.v. injection of each drug and the reported distribution of P-glycoprotein transporter for the drug was histochemically examined. Immunocytochemical studies for DX or DR using monoclonal antibody that equally detects both drugs, as well as conventional...

article

Immunocytochemistry for bestatin and its application to drug accumulation studies in rat intestine and kidney

Kunio Fujiwara, Masashi Shin, Yohei Yoshizaki, Tsubasa Miyazaki, more

Journal of Molecular Histology > 2011 > 42 > 6 > 589-596

The in vivo role of transporters in drug disposition, in the context of other transporters, and metabolism has not been established. We prepared an anti-bestatin serum against bestatin conjugated to albumin with glutaraldehyde (GA). The antiserum was specific for GA-conjugated bestatin and weakly reacted with free bestatin, but no reaction occurred with structurally unrelated compounds according to...

article

Decreased renal accumulation of aminoglycoside reflects defective receptor-mediated endocytosis in cystic fibrosis and Dent’s disease

Claudia Raggi, Kunio Fujiwara, Teresinha Leal, François Jouret, more

Pflügers Archiv - European Journal of Physiology > 2011 > 462 > 6 > 851-860

The clinical use of aminoglycoside (AG) antibiotics is limited by their renal toxicity, which is caused by drug accumulation in proximal tubule (PT) cells. Clinical studies reported that renal clearance of AG is enhanced in cystic fibrosis (CF) patients, which might reflect the role of CFTR in PT cell endocytosis. In order to assess the role of chloride transporters on the renal handling of AG, we...

article

Differences in accumulation of anthracyclines daunorubicin, doxorubicin and epirubicin in rat tissues revealed by immunocytochemistry

Masashi Shin, Hayato Matsunaga, Kunio Fujiwara

Histochemistry and Cell Biology > 2010 > 133 > 6 > 677-682

Although anthracycline antibiotics daunorubicin (DR), doxorubicin (DX), and epirubicin (ER) possess minor differences in their chemical structures, large differences are noted in their clinical use, as well as in cellular and plasma pharmacokinetic parameters in vivo. Immunocytochemistry for DR, DX, or ER was developed using an anti-DR monoclonal antibody (ADM-1-11), which has been demonstrated to...

article

Daunomycin accumulation and induction of programmed cell death in rat hair follicles

Masashi Shin, Lars-Inge Larsson, David M. Hougaard, Kunio Fujiwara

Cell and Tissue Research > 2009 > 337 > 3 > 429-438

The anthracycline antibiotic daunomycin (DM) is useful for the treatment of leukemia but has side-effects such as alopecia. Using immunocytochemistry, we show that, after a single i.v. injection, DM accumulates in the nuclei of matrix cells and in the outer root sheath of hair follicles. DM-positive matrix cells are detectable up to 48 h after injection and exhibit a characteristic granular morphology,...

article

Modulatory effects of histamine on cat carotid body chemoreception

Rodrigo Del Rio, Esteban A. Moya, Cecilia S. Koenig, Kunio Fujiwara, more

Respiratory Physiology & Neurobiology > 2008 > 164 > 3 > 401-410

Histamine has been proposed to be an excitatory transmitter between the carotid body (CB) chemoreceptor (glomus) cells and petrosal ganglion (PG) neurons. The histamine biosynthetic pathway, its storage and release, as well as the presence of histamine H1, H2 and H3 receptors have been found in the CB. However, there is only indirect evidence showing the presence of histamine in glomus cells, or weather...

article

Immunocytochemical demonstration of polyamines in nucleoli and nuclei

Masashi Shin, Hiromichi Nakamuta, Naoko Oda-Ueda, Lars-Inge Larsson, more

Histochemistry and Cell Biology > 2008 > 129 > 5 > 659-665

Although biochemical studies have shown that polyamines (PAs) occur in the nucleus, only few studies have examined the intranuclear distribution of these organic cations. By immunocytochemistry, we have previously demonstrated that PAs are located in ribosomes. We now show that PAs also are present in both nucleoli and nuclei of a variety of cell types. Detection of nucleolar and nuclear PAs required...

article

Polyamines in spermatocytes and residual bodies of rat testis

Masashi Shin, Lars-Inge Larsson, Kunio Fujiwara

Histochemistry and Cell Biology > 2007 > 127 > 6 > 649-655

Immunocytochemistry for polyamines in the rat testis revealed intense staining of small bodies close to the lumen of seminiferous tubules of spermatogenic stage VII and VIII as well as of spermatocytes. Methyl green-pyronin and propidium iodide staining combined with DNase or RNase predigestion showed that the small bodies contained RNA, but not DNA and double fluorescence staining showed that polyamines...

article

Distribution of anticancer antibiotic daunomycin in the rat heart and kidney revealed by immunocytochemistry using monoclonal antibodies

Kunio Fujiwara, Masashi Shin, David M. Hougaard, Lars-Inge Larsson

Histochemistry and Cell Biology > 2007 > 127 > 1 > 69-77

Two monoclonal antibodies (ADM-1-11 and 79-31 mAbs) were raised against daunomycin (DM) conjugated to bovine serum albumin via the cross-linker N-(gamma-maleimidobutyryloxy)succinimide. The monoclonal antibodies (mAbs) specifically detected DM as well as its analogs doxorubicin and epirubicin, but did not react with other anticancer antibiotics, including pepleomycin, mitomycin C, and actinomycin...

article

Immunocytochemical studies on the distribution pattern of daunomycin in rat gastrointestinal tract

Koji Ohara, Masashi Shin, Hiromichi Nakamuta, Lars-Inge Larsson, more

Histochemistry and Cell Biology > 2007 > 128 > 3 > 285-290

The cancer drug daunomycin is used in treatment of leukemia but possesses severe side effects that involve the gastrointestinal tract. We therefore used a newly developed immunocytochemical procedure to determine the distribution of DM in the gastrointestinal tracts of rats after i.v. injection. Two hours after injection, DM was diffusely distributed in nuclei and most parts of the cytoplasm of intestinal...

article

Improved immunocytochemical detection of daunomycin

Koji Ohara, Masashi Shin, Lars-Inge Larsson, Kunio Fujiwara

Histochemistry and Cell Biology > 2007 > 127 > 6 > 603-608

Improved immunocytochemical (ICC) detection of the anthracycline anticancer antibiotic daunomycin (DM) has been achieved by use of hydrogen peroxide oxidation prior to ICC staining for DM. The new method greatly enhanced the localization of DM accumulation in cardiac, smooth and skeletal muscle of rats after a single i.v. dose of the drug. DM accumulated in the nuclei as well as in the sarcoplasm,...

article

Immunohistochemical demonstration of the distribution of chloroquine (CQ) and its metabolites in CQ-poisoned mice

Ako Koreeda, Kosei Yonemitsu, Hiroe Kohmatsu, Sohtaro Mimasaka, more

Archives of Toxicology > 2007 > 81 > 7 > 471-478

Chloroquine (CQ) distribution in tissues of acutely poisoned mice was demonstrated by immunohistochemistry using anti-CQ polyclonal antibodies (PAC). PAC recognized 4-amino-7-chloro-quinoline structure and sufficiently reacted with CQ and CQ’s metabolite bisdesethyl-chloroquine. In the brain, CQ and its metabolites (CQs) localized in the region of the choroids plexus, indicating an important role...

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Search results for: Kunio Fujiwara

High-resolution separation of neodymium and dysprosium ions utilizing extractant-impregnated graft-type particles

Expression and distribution patterns of spermine, spermidine, and putrescine in rat hair follicle

A simple in situ preconcentration method for the simultaneous determination of major and minor alkali and alkaline-earth metals in natural waters using iminodiacetate-grafted fiber

On boron turnover in plant–litter–soil system

Role of polyamines at the G1/S boundary and G2/M phase of the cell cycle

Antimicrobial fabric adsorbed iodine produced by radiation-induced graft polymerization

An in vivo role of Mrp2 in the rat hepatocytes by immunocytochemistry for amoxicillin using the transporter-deficient EHBR

Distribution study of peplomycin in rat kidney revealed by immunocytochemistry using monoclonal antibodies

A probable relationship between characteristic accumulation of doxorubicin and P-glycoprotein transporter in rat liver

Immunocytochemistry for bestatin and its application to drug accumulation studies in rat intestine and kidney

Decreased renal accumulation of aminoglycoside reflects defective receptor-mediated endocytosis in cystic fibrosis and Dent’s disease

Differences in accumulation of anthracyclines daunorubicin, doxorubicin and epirubicin in rat tissues revealed by immunocytochemistry

Daunomycin accumulation and induction of programmed cell death in rat hair follicles

Modulatory effects of histamine on cat carotid body chemoreception

Immunocytochemical demonstration of polyamines in nucleoli and nuclei

Polyamines in spermatocytes and residual bodies of rat testis

Distribution of anticancer antibiotic daunomycin in the rat heart and kidney revealed by immunocytochemistry using monoclonal antibodies

Immunocytochemical studies on the distribution pattern of daunomycin in rat gastrointestinal tract

Improved immunocytochemical detection of daunomycin

Immunohistochemical demonstration of the distribution of chloroquine (CQ) and its metabolites in CQ-poisoned mice

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