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To evaluate the associations between proper protamine incorporation and DNA methylation at imprinted loci.Experimental research study.Research laboratory.Three populations were tested—abnormal protamine patients, oligozoospermic patients, and fertile donors.The CpG methylation patterns were examined at seven imprinted loci sequenced: LIT1, MEST, SNRPN, PLAGL1, PEG3, H19, and IGF2.The DNA methylation...