Search results for: Liliana Anjos

Items from 1 to 11 out of 11 results

article

Short- and long-term effects on growth and expression patterns in response to incubation temperatures in Senegalese sole

Carlos Carballo, Joana Firmino, Liliana Anjos, Soraia Santos, more

Aquaculture > 2018 > 495 > C > 222-231

In this study, the short- and long-term effects of embryo incubation temperatures (16, 18 and 20 °C) on development and growth of the flatfish Senegalese sole (Solea senegalensis) was determined by investigating the expression patterns of the epigenetic regulators DNA methyltransferases (dnmt) and histone 3 (H3) and genes belonging to the retinoic acid (RA), insulin-like growth factors (IGFs) and...

article

Structural and functional maturation of skin during metamorphosis in the Atlantic halibut (Hippoglossus hippoglossus)

Ricardo N. Alves, Kristina S. Sundell, Liliana Anjos, Henrik Sundh, more

Cell and Tissue Research > 2018 > 372 > 3 > 469-492

To establish if the developmental changes in the primary barrier and osmoregulatory capacity of Atlantic halibut skin are modified during metamorphosis, histological, histochemical, gene expression and electrophysiological measurements were made. The morphology of the ocular and abocular skin started to diverge during the metamorphic climax and ocular skin appeared thicker and more stratified. Neutral...

article

Molecular cloning and functional characterization of a monoterpene synthase isolated from the aromatic wild shrub Thymus albicans

Alexandra Filipe, João C.R. Cardoso, Graça Miguel, Liliana Anjos, more

Journal of Plant Physiology > 2017 > 218 > C > 35-44

The essential oil of Thymus albicans Hoffmanns. & Link, a native shrub from the Iberian Peninsula, is mainly composed of monoterpenes. In this study, a 1,8-cineole synthase was isolated from the 1,8-cineole chemotype. A partial sequence that lacked the complete plastid transit peptide but contained an extended C-terminal when compared to other related terpene synthases was generated by PCR and...

article

Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.)

Ana Patrícia Mateus, Liliana Anjos, João R. Cardoso, Deborah M. Power

Molecular Immunology > 2017 > 87 > C > 267-283

Scale removal in fish triggers a damage-repair program to re-establish the lost epidermis and scale and an associated local immune response. In mammals, chronic stress is known to delay wound healing and to modulate the cutaneous stress axis, but this is unstudied in teleost fish the most successful extant vertebrates. The present study was designed to test the hypothesis that chronic stress impairs...

article

Cover Image, Volume 85, Issue 2

Liliana Anjos, Isabel Morgado, Marta Guerreiro, João C. R. Cardoso, more

Proteins: Structure, Function, and Bioinformatics > 85 > 2 > C1 - C1

The cover image, by Isabel Morgado et al., is based on the Article Cartilage acidic protein 1, a new member of the beta‐propeller protein family with amyloid propensity, DOI: 10.1002/prot.25210.

article

Cartilage acidic protein 1, a new member of the beta‐propeller protein family with amyloid propensity

Liliana Anjos, Isabel Morgado, Marta Guerreiro, João C. R. Cardoso, more

Proteins: Structure, Function, and Bioinformatics > 85 > 2 > 242 - 255

Cartilage acidic protein1 (CRTAC1) is an extracellular matrix protein of chondrogenic tissue in humans and its presence in bacteria indicate it is of ancient origin. Structural modeling of piscine CRTAC1 reveals it belongs to the large family of beta‐propeller proteins that in mammals have been associated with diseases, including amyloid diseases such as Alzheimer's. In order to characterize the structure/function...

article

PTHrP-induced modifications of the sea bream (Sparus auratus) vertebral bone proteome

Liliana Anjos, Ana S. Gomes, Begoña Redruello, Richard Reinhardt, more

General and Comparative Endocrinology > 2013 > 191 > Complete > 102-112

Endocrine factors play an essential role in the formation and turnover of the skeleton in vertebrates. In the present study sea bream vertebral bone transcripts for PTH1R and PTH3R were identified and the action of intermittent administration of parathyroid hormone related protein (PTHrP) on the proteome of vertebral bone was analysed. Treatment of immature sea bream (Sparus auratus, n=6) for 5days...

article

Cartilage Acidic Protein 2 a hyperthermostable, high affinity calcium-binding protein

Liliana Anjos, Ana S. Gomes, Eduardo P. Melo, Adelino V. Canário, more

BBA - Proteins and Proteomics > 2013 > 1834 > 3 > 642-650

Cartilage Acidic Protein 2 (CRTAC2) is a novel protein present from prokaryotes to vertebrates with abundant expression in the teleost fish pituitary gland and an isoform of CRTAC1, a chondrocyte marker in humans. The two proteins are non-integrins containing N-terminal integrin-like Ca ²⁺ -binding motifs and their structure and function remain to be assigned. Structural studies of recombinant...

article

Four stanniocalcin genes in teleost fish: Structure, phylogenetic analysis, tissue distribution and expression during hypercalcemic challenge

Vanessa Schein, João C.R. Cardoso, Patrícia I.S. Pinto, Liliana Anjos, more

General and Comparative Endocrinology > 2012 > 175 > 2 > 344-356

Stanniocalcin (STC), first isolated from the corpuscles of Stannius (CS) of teleost fishes and a systemic regulator of mineral metabolism, is present in all vertebrates as two isoforms, STC1 and STC2, encoded by separate genes. Here we show that the genome of Tetraodon nigroviridis, and other teleosts, possess duplicate genes for each STC isoform, designated stc1-a and -b, and stc2-a and -b. Stc1-a...

article

CRTAC1 homolog proteins are conserved from cyanobacteria to man and secreted by the teleost fish pituitary gland

Begoña Redruello, Bruno Louro, Liliana Anjos, Nádia Silva, more

Gene > 2010 > 456 > 1-2 > 1-14

Cartilage acidic protein 1 (CRTAC1) gene expression is used as a marker for chondrocyte differentiation in stem cell-based tissue engineering. It is also transcribed outside the skeleton where at least two different transcripts are expressed in lung and brain. In the pituitary gland of the teleost fish sea bream Sparus auratus, we have found a transcript with a high degree of sequence identity to...

article

Isolation of Atlantic halibut pituitary hormones by continuous-elution electrophoresis followed by fingerprint identification, and assessment of growth hormone content during larval development

Ingibjörg Eir Einarsdóttir, Liliana Anjos, Jon Hildahl, Björn Thrandur Björnsson, more

General and Comparative Endocrinology > 2007 > 150 > 2 > 355-363

Continuous-elution electrophoresis (CEE) has been applied to separate putative hormones from adult Atlantic halibut pituitaries. Soluble proteins were separated by size and charge on Model 491 Prep Cell (Bio-Rad), where the homogenate runs through a cylindrical gel, and protein fractions are collected as they elute from the matrix. Protein fractions were assessed by SDS–PAGE and found to contain purified...

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