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Hydrolysis of phosphoric acid-impregnated steam-treated sugarcane bagasse was pre-optimized using a face-centered central composite design in which the process variables were the substrate total solids (TS, %), agitation intensity (AI, rpm) and enzyme loading (EL, gg−1). Pretreatment was carried out at 180°C for 10min using cane bagasse with 50wt% moisture content containing 9.5mg of H3PO4 per gram...