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To accurately determine mitochondrial DNA (mtDNA) levels in human blastocysts.Retrospective analysis.IVF clinic.A total of 1,396 embryos derived from 259 patients.Blastocyst-derived trophectoderm biopsies were tested by next-generation sequencing (NGS) and quantitative real-time polymerase chain reaction (qPCR).For each sample the mtDNA value was divided by the nuclear DNA value, and the result was...