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Contact analysis of mechanical interface has been an important engineering problem. Based on the interaction principle between ultrasonic wave and imperfect interface, experimental method on contact area and distribution of steel-steel interface with pulse-echo technique is presented in this paper. In the method, ultrasonic echo signal being denoised and transformed with Fourier transform to get amplitude...
To interfere with the endogenous gene encoding squalene synthase (SS) responsible for sterol biosynthesis, an antisense SS cDNA (asSS) was introduced into the genome of A. annua. Following the decline of SS mRNA and synchronous reduction of total sterols, mRNA coding for amorpha-4,11-diene synthase (ADS) tremendously elevated, whereas mRNAs for sesquiterpene cyclases encompassing 8-epicedrol synthase...
To elucidate the fine-tuned temporal and spatial modulation of artemisinin production in annual wormwood (Artemisia annua), we conducted enzyme-linked immunosorbent assay-based immunoquantification of three key enzymes involved in artemisinin biosynthesis, amorpha-4,11-diene synthase (ADS), cytochrome P450 monooxygenase (CYP71AV1), and cytochrome P450 reductase (CPR), in various tissues and under...
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