Search results for: Toru Takada

Items from 1 to 14 out of 14 results

article

Delayed notochordal cell disappearance through integrin α5β1 mechanotransduction during ex‐vivo dynamic loading‐induced intervertebral disc degeneration

Yutaro Kanda, Takashi Yurube, Yusuke Morita, Yoshiki Takeoka, more

Journal of Orthopaedic Research® > 39 > 9 > 1933 - 1944

The loss of nucleus pulposus (NP) notochordal cells is one of the key initial hallmarks of age‐related intervertebral disc degeneration. Although the transmembrane mechanoreceptor integrin α5β1 is important in the process of disc degeneration, the relationship between integrin α5β1 and notochordal cell disappearance remains unclear. The purpose of this study was to elucidate the role of integrin α5β1...

article

A novel topical treatment for bone metastases using a gelatin hydrogel incorporating cisplatin as a sustained release system

Yutaro Kanda, Kenichiro Kakutani, Takashi Yurube, Zhongying Zhang, more

Journal of Orthopaedic Research® > 39 > 3 > 525 - 535

Management of bone metastasis is becoming increasingly important. Thus, local and systemic treatment options have been developed for control. Although systemic administration of anticancer agents is effective for bone metastasis, it is often stopped because of poor general conditions or side effects. Therefore, it is highly desirable to develop a more effective and safer local treatment for bone metastasis...

article

Quality of life and cost-utility of surgical treatment for patients with spinal metastases: prospective cohort study

Shingo Miyazaki, Kenichiro Kakutani, Yoshitada Sakai, Yasuo Ejima, more

International Orthopaedics > 2017 > 41 > 6 > 1265-1271

Purpose Palliative surgery for patients with spinal metastasis provides good clinical outcomes. However, there have been few studies on quality of life (QOL) and cost-utility of this surgery. We aimed to elucidate QOL and cost-utility of surgical treatment for spinal metastasis. Methods We prospectively analyzed 47 patients with spinal metastasis from 2010 to 2014 who had a surgical indication....

article

Surgical debridement with retention of spinal instrumentation and long-term antimicrobial therapy for multidrug-resistant surgical site infections after spinal surgery: a case series

Shingo Miyazaki, Kenichiro Kakutani, Koichiro Maeno, Toru Takada, more

International Orthopaedics > 2016 > 40 > 6 > 1171-1177

Purpose Post-operative surgical site infection (SSI) is one of the most significant complications after instrumented spinal surgery. However, implant retention feasibility for early-onset multidrug-resistant SSI is still controversial. We aimed to verify our therapeutic strategy, surgical debridement with implant retention and long-term antimicrobial therapy for post-operative early-onset multidrug-resistant...

article

Functional impact of integrin α5β1 on the homeostasis of intervertebral discs: a study of mechanotransduction pathways using a novel dynamic loading organ culture system

Takuto Kurakawa, Kenichiro Kakutani, Yusuke Morita, Yuki Kato, more

The Spine Journal > 2015 > 15 > 3 > 417-426

Intervertebral disc (IVD) degeneration, a major cause of low back pain, is considered to be induced by daily mechanical loading. Mechanical stress is widely known to affect cell survival and extracellular matrix metabolism in many cell types. Although the involvement of integrin α5β1 transmembrane mechanoreceptor in IVD degeneration has been reported, the precise function of integrin α5β1 remains...

article

Recombinant human SIRT1 protects against nutrient deprivation-induced mitochondrial apoptosis through autophagy induction in human intervertebral disc nucleus pulposus cells

Shingo Miyazaki, Kenichiro Kakutani, Takashi Yurube, Koichiro Maeno, more

Arthritis Research & Therapy > 2015 > 17 > 1 > 1-12

Introduction Nutrient deprivation is a likely contributor to intervertebral disc (IVD) degeneration. Silent mating type information regulator 2 homolog 1 (SIRT1) protects cells against limited nutrition by modulation of apoptosis and autophagy. However, little evidence exists regarding the extent to which SIRT1 affects IVD cells. Therefore, we conducted an in vitro study using human IVD nucleus pulposus...

article

A rat tail temporary static compression model reproduces different stages of intervertebral disc degeneration with decreased notochordal cell phenotype

Hiroaki Hirata, Takashi Yurube, Kenichiro Kakutani, Koichiro Maeno, more

Journal of Orthopaedic Research® > 32 > 3 > 455 - 463

The intervertebral disc nucleus pulposus (NP) has two phenotypically distinct cell types—notochordal cells (NCs) and non‐notochordal chondrocyte‐like cells. In human discs, NCs are lost during adolescence, which is also when discs begin to show degenerative signs. However, little evidence exists regarding the link between NC disappearance and the pathogenesis of disc degeneration. To clarify this,...

article

Fas ligand plays an important role for the production of pro‐inflammatory cytokines in intervertebral disc nucleus pulposus cells

Junya Yamamoto, Koichiro Maeno, Toru Takada, Kenichiro Kakutani, more

Journal of Orthopaedic Research® > 31 > 4 > 608 - 615

It is suggested that pro‐inflammatory cytokines, which are produced by interaction of the intervertebral nucleus pulposus cells and macrophages, may be linked to the cause of pain of the intervertebral disc herniation. This study carries out the in vitro experiments to examine the mechanism, with the use of the co‐culture of an immortalized cell line of nucleus pulposus of the human intervertebral...

article

Intervertebral disc and macrophage interaction induces mechanical hyperalgesia and cytokine production in a herniated disc model in rats

Toru Takada, Kotaro Nishida, Koichiro Maeno, Kenichiro Kakutani, more

Arthritis & Rheumatology > 64 > 8 > 2601 - 2610

ObjectiveThe expression of proinflammatory factors such as tumor necrosis factor α (TNFα), interleukin‐6 (IL‐6), IL‐8, and prostaglandin E₂ (PGE₂) is significantly correlated with the symptoms of herniated disc disease. Among the different types of immune cells, macrophages are frequently noted in the herniated disc tissue. We undertook this study to clarify the interaction of the intervertebral disc...

article

Modified house‐keeping gene expression in a rat tail compression loading‐induced disc degeneration model

Takashi Yurube, Toru Takada, Hiroaki Hirata, Kenichiro Kakutani, more

Journal of Orthopaedic Research® > 29 > 8 > 1284 - 1290

House‐keeping genes (HKGs) are generally used as endogenous controls for molecular normalization in quantitative PCR analysis. However, whether all the so‐called HKGs are useful for intervertebral disc research is controversial. Our objective was, using a prevalidated rat tail static compression loading‐induced disc degeneration model, to clarify the feasibility of common HKGs for gene‐quantification...

article

Matrix metalloproteinase (MMP)‐3 gene up‐regulation in a rat tail compression loading‐induced disc degeneration model

Takashi Yurube, Kotaro Nishida, Teppei Suzuki, Shuichi Kaneyama, more

Journal of Orthopaedic Research® > 28 > 8 > 1026 - 1032

The rodent static compression loading‐induced disc degeneration model still has important gaps among the radiographic, magnetic resonance imaging (MRI), and histological schemes and the acute and chronic expression of catabolic genes such as matrix metalloproteinase (MMP)‐3. Our objectives were to assess the validity of a rat tail two‐disc static compression model and to elucidate a representative...

article

Sustained long-term RNA interference in nucleus pulposus cells in vivo mediated by unmodified small interfering RNA

Teppei Suzuki, Kotaro Nishida, Kenichiro Kakutani, Koichiro Maeno, more

European Spine Journal > 2009 > 18 > 2 > 263-270

RNA interference (RNAi) technology has recently emerged as an important biological strategy for gene silencing. Previously, the efficacies of RNAi in cultured nucleus pulposus cells in vitro have been reported. However, RNAi in the disc in vivo has never been reported. Therefore, the aims of the present study were to establish a method for RNAi in the disc in vivo and to evaluate the applicability...

article

Fas ligand expression on human nucleus pulposus cells decreases with disc degeneration processes

Shuichi Kaneyama, Kotaro Nishida, Toru Takada, Teppei Suzuki, more

Journal of Orthopaedic Science > 2008 > 13 > 2 > 130-135

Background The intervertebral disc has been reported to be an immunologically privileged environment, possibly mediated by Fas ligand (FasL) expression. On the other hand, recent studies have shown the infiltration of host immune cells into the degenerated disc, which may indicate the failure of the immune-privilege feature of the disc with degeneration. However, the relationship between FasL expression...

article

Immunosuppression by Anti-ICAM-1 and Anti-LFA-1 Monoclonal Antibodies of Free and Vascularized Skin Allograft Rejection

Tetsuya Iwata, Yuzuru Kamei, Shigeru Esaki, Toru Takada, more

Immunobiology > 1996 > 195 > 2 > 160-171

Immunosuppression by anti-adhesion molecule antibody of free or vascularized skin allograft rejection was investigated in rats. Lewis (LEW, RT1 ¹ ) rats were used as donors and Fisher (F344, RT1 ^1v1 ) rats as the recipients. When F344 rats were treated intraperitoneally (i.p.) with anti-intercellular adhesion molecule-1 (ICAM-1) mAb (1A29) (3 mg/kg/day) and anti-leukocyte function-associated...

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Keywords

ANIMAL MODEL (4)
INTERVERTEBRAL DISC DEGENERATION (4)
SPINE (3)
APOPTOTIC CELL DEATH (2)
NOTOCHORDAL CELL PHENOTYPE (2)
A DISINTEGRIN AND METALLOPROTEINASE (ADAM) 10 (1)
ANTIMICROBIAL THERAPY (1)
ANTITUMOR DRUG (1)
BONE METASTASIS (1)
CDDP (1)
COMPRESSION (1)
COST EFFECTIVE (1)
COST-UTILITY (1)
DISC DEGENERATION (1)
DYNAMIC LOADING (1)
EUROQOL 5D (1)
FAS LIGAND (FASL) (1)
GELATIN HYDROGEL (1)
HOUSE‐KEEPING GENE (1)
IMPLANT RETENTION (1)
INCREMENTAL COST-UTILITY RATIO (1)
INTEGRIN (1)
INTEGRIN MECHANOTRANSDUCTION (1)
INTERVERTABRAL DISC (1)
INTERVERTEBRAL DISC (1)
INTERVERTEBRAL DISC HERNIATION (1)
MATRIX METALLOPROTEINASE (MMP)‐3 (1)
MECHANICAL LOAD (1)
MECHANORECEPTOR (1)
MULTIDRUG RESISTANCE (1)
NUCLEUS PULPOSUS CELL (1)
ORGAN CULTURE (1)
PRO‐INFLAMMATORY CYTOKINES (1)
QUALITY OF LIFE (1)
QUALITY-ADJUSTED LIFE YEARS (1)
REAL‐TIME RT‐PCR MRNA‐QUANTIFICATION (1)
RNA INTERFERENCE (RNAI) (1)
SPINAL METASTASIS (1)
SURGICAL DEBRIDEMENT (1)
SURGICAL SITE INFECTION (1)
SURGICAL TREATMENT (1)
SUSTAINED RELEASE (1)
ULTRASOUND GENE THERAPY (1)
UNMODIFIED SIRNA (1)
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Wiley (7)
Springer (5)
Elsevier (2)

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Search results for: Toru Takada

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