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The binding of the lac repressor and the lac repressor N-terminal headpiece to a mini-operator with a hairpin loop made of a hexaethylene glycol chain was investigated using circular dichroism spectroscopy. The Iac repressor's headpiece binds to the modified mini-operator with the same affinity as to a mini-operator of the same sequence without the hexaethylene glycol loop. The conformational effect...
The conformation of an octathymidylate synthesized with the α-anomer of thymidine has been studied as a function of temperature using circular dichroism. At high ionic strength a sigmoidal melting profile indicates the formation of a double-stranded structure. This duplex structure is stabilized by addition of MgCl 2 . The thermodynamic parameters ΔH and ΔS corresponding to the formation of...
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