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Introduction
Lymphocyte subset enumeration by flow cytometry is important for the therapeutic monitoring of a range of conditions. However, current bead‐based methodologies do not produce metrologically traceable results. Here we compare an established bead‐based methodology with a volumetric‐based system traceable to an internationally recognised reference method.
Method
A total of 118 samples...
Background
Detection of del(17p) in myeloma is generally performed by fluorescence in situ hybridization (FISH) on a slide with analysis of up to 200 nuclei. The small cell sample analyzed makes this a low precision test. We report the utility of an automated FISH method, called “immuno‐flowFISH”, to detect plasma cells with adverse prognostic risk del(17p) in bone marrow and blood samples of patients...