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Gold nanocages (AuNCs) were synthesized via galvanic replacement method and characterized by TEM and UV-Vis-NIR. In order to investigate the adjuvant potential of AuNCs, AuNC-formulated recombinant Hepatitis B surface antigen (HBsAg) preparations were used. First, the adsorption efficiency and cumulative release percentage of newly created HBsAg-AuNC formulations were characterized. Then, their adjuvant...
In this paper, a novel Micro Electro Mechanical System (MEMS) based cantilever beam of cross section (400×150×1)μm is presented for the detection of Metronidazole (MZN) which can be used in food processing industry to detect its toxicity level in meat. MZN is a drug that is proven to be carcinogenic if used as feed addictive for cattle breeding or aquafarming. Molecular imprinted Gold cantilever beam...
In recent years, nanocars with p-carborane wheels have been a subject of interest as an artificial molecular machine. Researchers aim to discover compositions that are easier to fabricate, efficient and are more stable on the surface. The p-carborane molecule has all these key elements, making it a viable choice as a nanocar wheel, and the mobility of a nanocar is heavily influenced by the motion...
Bacteriophages are ideal recognition elements for bacterium because of specificity and high affinity in the field of biosensors. The effect of bimolecular immobilization directly influences the performance of biosensors, so orientation immobilization of bacteriophages is of great importance to the biosensors. This article reviews the recent progress in biosensors that used a variety of strategies...
In this work time- and frequency-resolved spectroscopic techniques are employed for the investigation of protein adsorption (protein corona formation) on gold nanoparticles. We will follow the interaction of ∼ 20 nm citrate-capped gold nanoparticles with the model protein bovine serum albumin under both static and flow conditions, to monitor in details the binding process in the early stages. In parallel...
Rapid and sensitive chemical sensing using microfluidic device equipped with sterically-bulky three-dimensional gold nanostructure (Au3D) was demonstrated. Au3D was expected as surface enhanced Raman scattering (SERS) active structure. Au3D was fabricated by convective self-assembly known as “coffee-ring” of the mixed solution of gold colloidal nanoparticle and polystyrene latex particle. SERS measurements...
A novel optical biosensor based on gold nanorods (GNRs) was designed and fabricated for simple, sensitive and competitive detection of aflatoxin B1 (AFB1). Free AFB1 target interacted with capture antibodies, competing with AFB1-BSA (Bovine serum albumin) conjugate modified GNRs. Aggregation of GNRs was observed if the amount of AFB1 target was not enough. As a result, an associated decrease in the...
Regarding the adsorption tower in the process of adsorption as a research object, based on the characteristics of gas flow and heat transfer in the process of adsorption, the mathematical model was established in the process of adsorption according to the theories of thermodynamics, fluid mechanics and heat transfer. Through numerical solution, the changes of temperature, pressure and adsorption capacity...
A study of the adsorption kinetics of 1-octanethiol and 1,8-octanedithiol has been carried out on gold electrode by cyclic voltammetry with different concentrations. Analysis of the kinetic data show that the adsorption kinetics of 1-octanethiol and 1,8-octanedithiol can be satisfactorily described by Diffusion controlled Langmuir(DCL) model, irrespective of concentration. The rate constants of 1-octanethiol...
This paper describes a glucose biosensor based on glucose oxidase (GOx) immobilized on porous ZnO/Au electrodes. The ZnO porous electrodes were fabricated by electrochemical deposition of zinc oxide on patterned Au electrodes using polystyrene (PS) spheres as templates. Uniform pore size and highly ordered ZnO pore arrangement were observed from SEM images. X-ray diffraction (XRD) patterns revealed...
Sensing applications in the petroleum industry pose unique challenges due to high viscosity and high refractive index of hydrocarbon fluids, which render many fluid-based techniques impractical. Real-time observation of adsorption, desorption, and competitive adsorption processes of long chain functionalized hydrocarbons onto activated cantilevers in static mode are shown. In addition, microcantilevers...
The fast and sensitive detections of chemicals adsorption onto solid substrates are of importance to many fields. Ordered arrays of gold nanoparticles with strong surface plasmon resonances allow directly visualized detection of molecular scale adsorptions of mercury vapor on solid substrates. The adsorbed species change local dielectric constants surrounding plasmonic nanoparticles, leading to shifts...
A new analysis method of gold in sulfate leaching solution is introduced in this paper. Hydrochloric acid is added into solution to decompose thiosulfate for quickly and effectively undermining the gold thiosulfate complex. In the medium of aqua fortis, gold is adsorbed and separated by mercapto group resin. After elution, isoamyl alcohol is used as extractant to enrich and separate Au3+ from solution...
This study presents combination of advantages in MEMS technology and molecular methods for detection of methicillin resistant gene, mecA, in Staphylococcus aureus. A disposable MEMS DNA biosensor was designed and fabricated for optical and electrochemical detections. Adsorption of DNA on gold electrode was examined theoretically and experimentally. In theoretical studies, Lennard Jones potential energies...
Nanosphere lithography has potential in many areas, and there have been many methods that deposit the colloidal suspension onto the substrate. However, it is important and still difficult to control the uniformity of the monolayer on a plane substrate. In this study, we have found a method for a uniform monolayer of polystyrene beads by confining the droplets with microwells. By simply controlling...
The protein adsorption is a crucial but complex phenomenon in many applications, like biosensors, biomaterials. The amount, orientation and biological activity of adsorbed proteins are heavily dependent upon the structure of protein, like widely-applied Bovine Serum Albumin (BSA), and the properties of the substrate. At some extent, to explore the physicochemical properties and dynamics interaction...
The self-assembly adsorption of bovine serum albumin (BSA) on the hybrid surface including the discontinuous gold nano film and silica under liquid condition was studied in situ by atomic force microscope (AFM) at room temperature. To investigate the adsorption of BSA on silica and gold nano film simultaneously, the surface with discontinuous gold nano film and visible blank block on silica by physical...
Two-photon-excited fluorescence (TPEF) enhancement of a semiconductor quantum dot (SQD) adsorbed on a Au nanoparticle (NP) was investigated theoretically. Surface plasmon resonance (SPR) enhancement of the exciting light and radiative rate of the SQD, and nonradiative energy transfer (NRET) from SQD to Au NP were taken into account. Compared to the unitary enhancement factor obtained from considering...
A novel nano Fe3O4-Au modified amperometric immunosensor for rapid separation-free determination of human immunodeficiency virus (HIV) p24 antigen in acquired immunodeficiency syndrome (AEDs) patients' serum is proposed. Firstly, one novel sebacato-bridged Cu(II) phen complex Cu(phen)L2 (referred to as CuL, H2L=sebacic acid; phen=1,10phenanthroline) was absorbed on the surface of glassy carbon electrode...
Self-assembled monolayer (SAM) techniques offer a new strategy for the immobilization of antibodies, antigens and DNA on the surface of quartz crystal electrodes. The quartz crystal microbalance (QCM) system provides a unique opportunity to monitor, in-situ and in real-time, the rate of adsorption of ethanthiol onto gold. The change in frequency resulting from the adsorption of ethanthiol molecules...
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