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The paper is devoted to the history and prospects of gene therapy, a new area of biotechnology that holds great promise for the treatment genetic disorders, cancer and dangerous infections such as HIV and hepatitis B. A brief history of gene therapy is described. The paper discusses the main lines of research in this area as well as the mechanisms, approaches, and techniques employed by gene therapy,...
MicroRNAs are short regulatory genes that regulate the gene expression in various cellular activities and pathways. Identification of microRNA target site is important for the understanding of pathogenesis and cellular functions. This study applies a computational approach to identify hsa-miR-122 target sites in hepatitis C virus isolate JFH-1. The minimum free energy, seed region complementarity,...
Protein therapeutics is used in the treatment of diabetes and various forms of cancer. A major concern is that repeated administration to patients often leads to undesirable antidrug antibodies with a wide range of life threatening consequences that induce immunogenicity or an adverse response of the immune system. The antibodies are generally thought triggered by the tendency of monomer protein molecules...
The Human Protein Atlas (HPA) is a repository of location patterns of about 11000 proteins within tissues and cell lines. In this work we summarize some of our current work on analyzing immunohistochemical images of proteins within 7 distinct tissues. Firstly, we present our efforts to analyze spatial point patterns of protein staining and determine protein subcellular location from image-derived...
Ovarian cancer is the leading cause of death in gynecological cancers. Carboplatinum-based therapy is the standard treatment choice for ovarian cancer. However, a majority of the patients develop resistance to carboplatinum fairly rapidly hence there is a clinical need for early predictors of carboplatinum resistance. While there are a few indicative gene markers, they have poor sensitivity and specificity...
We explore whether antisense oligodeoxy-nucleotides (ASODN) targeting survivin gene can reverse apoptotic resistance induced by cisplatin in human lung adenocarcinoma cells. A549 / CDDP cell lines were cultured routinely with RPMI-1640 medium. Survivin ASODN mediated by cytofectin was transfected into the A549 / CDDP cells. The influence of ASODN transfection on apoptosis was determined by Diphenylamine...
In this research, we have extended the use of Kernel Dimensionality Reduction (KDR) in the context of semi supervised learning in particular for micro-array DNA clustering application. We have proposed a new model call K-means-KDR for survival analysis which we aimed to improve the genes classification performance and study the dimension of effective subspaces in cancer patient survival analysis....
Objective: To study the relationship between the methylation status of multi-drug resistance protein (MRP) gene and the expression of its mRNA and protein in lung cancer cell lines. Methods: Human lung cell line WI-38.1ung adenocarcinoma cell line SPCA-1 and its drug-resistant cells induced by different concentrations of doxorubicin were treated with restriction endonuclease Eco47III. The methylation...
The present study shows that combinations of cisplatin and YH12 produce sequence- and concentration-dependent synergism in human ovarian cancer cell lines A2780 and A2780cisR. Addition of YH12 followed by cisplatin 4 h later or addition of the two compounds at the same time produces synergism whereas the addition of cisplatin first followed by YH12 4 h later results into antagonism. If the results...
This study was comparison of MGMT and ERBB2 expression in human gliomas. RT-PCR method was used to determine the mRNA levels of MGMT and ErbB2 gene in 42 clinical glioma specimens,There was significant concordance between the expression of MGMT and ErbB2 (p < 0.01). MGMT and ErbB are highly remarkably related each other. The results suggest that there may be in relationship among MGMT and ErbB2...
Objectives: To compare anti-tumor activities of different extracts from the medicinal plants Pinellia ternata and its allied species.Methods: MTT cell proliferation assay was used to evaluate the cytotoxicity of the extracts on Bel-7402, HT-29, Hela cells. FCM assay and DNA fragmentation analysis were used to detect cell apoptosis. Immunohistochemical SABC method was performed for detection of the...
Effective treatment of chronic hepatitis B virus (HBV) patients aims to prevent progression of chronic hepatitis B to cirrhosis, hepatocellular carcinoma, and eventually death. The use of mathematical modelling to explain the anti-HBV infection therapy has made a significant contribution for understanding the dynamics of host cells, viruses, the immune system. Chinese herbs are playing important roles...
An important goal for prostate cancer therapy is to identify novel mechanisms of androgen signaling that may provide new targets for androgen blockade therapy. Androgen regulated target genes continue to be identified, and include genes with regulatory regions containing 1) classical dimeric androgen receptor elements, or 2) sites for other transcription factors that tether androgen receptor to a...
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