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This article provides a brief overview and background on current biosensor designs for applications in genomics, proteomics and metabolomics. As research continues in many different aspects of biosensor design, new and better ways to recognize and detect low concentrations of a wide variety of different biomolecules are being developed for various applications. Biosensors can offer a simpler, faster...
The Mn2O3-Ag nanofibers were fabricated by electrospinning PVP sol-gel solution containing Mn(NO3)2 and AgNO3, followed by calcination in air at 500 °C for 3 h. The as-prepared Mn2O3-Ag nanofibers were employed as the immobilization matrix for glucose oxidase (GOD) to construct an amperometric sensor for glucose detection in pH 7 phosphate buffer. The Mn2O3-Ag-GOD modified electrode demonstrated fast...
A flexible substrate electrochemical biosensor was successfully printed on a polyethylene terephthalate (PET) film with silver (Ag) electrodes using silver nanoparticle based ink. The electrochemical impedance spectroscopy (EIS) response of the printed sensor for detecting low concentrations of bio/chemical species revealed a very high sensitivity at pico molar (pM) concentration levels of D-Proline,...
High density gold nanoparticles (GNPs) on indium tin oxide (ITO) film coated glass have been prepared by electrochemical deposition from KAu(CN)2 solution. In this study, suitable applied potential ranges and deposition cycles are chosen to achieve high density GNPs on ITO surface. The potential utility of the high density GNP/ITO substrate are investigated. The superoxide dismutase (SOD) was immobilized...
This paper reports a rapid, simple and direct method for the synthesis of gold nanoparticles (AuNPs) in polypyrrole (PPy) film, which provides a biocompatible matrix for the strong adsorption of antibodies. The electrochemical synthesis of AuNPs/PPy composite on gold or carbon electrode occurs within 20 minutes, and does not involve addition of any nanoparticles or reducing agent. This method is compatible...
In recent years, quartz crystal microbalance (QCM) plays an important role in many biological detections, and the enhancement of a single strain DNA probe linked to the sensor surface is of crucial importance in DNA molecule recognition. By means of nanogold modification and mass amplification, a QCM biosensor for Escherichia coli O157:H7 DNA detection was explored and developed in this work. As a...
We have constructed a piezoelectric sensor matrix with the capability of measuring the flexibility of immunoglobulin. The matrix consisted of 6 independent quartz crystal microbalance (QCM) coated with gold nanoparticles (GNP) of 3.5, 5, 12, 17, and 37 nm, and BSA. The detection was performed simultaneously. Antiserum against GNP bound to QCMs coated with 3.5 nm GNP and maximized for 5 nm GNP-coated...
Poly (sodium 4-styrenesulfonate) (PSS) was grafted onto carbon black surface by ambient surface-initiated atom radical transfer polymerization. And the sensors of screen printed carbon electrode based on CB-g-PSS immobilized with glucose oxidase were completed. The effect of CB-g-PSS on the response of amperometric glucose oxidase electrode for glucose was examined. The experimental results indicate...
The considerable effect of titanium dioxide (TiO2) nanoparticles and UV irradiation on the biomolecular binding behavior of anticancer drug doxorubicin has been studied by using electrochemical methods. Our observations demonstrate that the combination of nano TiO2 and UV irradiation could facilitate the respective DNA binding affinity of anticancer agent and significantly enhance the detection sensitivity...
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