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Purpose: The electron-transfer (ET) events in SQR, which may involve the 4Fe-4S cluster and/or the b-heme, and the mechanism of quinone reduction are not well understood. The experiments reported here are designed to gain greater insight into these issues.Methods: SQR was purified from Paracoccus denitrificans. Experiments involved EPR and transient absorption (TA) spectroscopy.Results: EPR experiments...
A model system to observe and investigate the transfer of Na+ ions between different internal compartments in suspension of liposomes was developed, and the exchange was followed by nuclear magnetic resonance spectroscopy. The experiments were performed under conditions of a Donnan equilibrium. Quantitative analysis of this three-site transmembrane exchange system allowed us to distinguish between...
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